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Anti-EGFR Antibody (6P389)
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Catalog No. TMAY-00024
Alias PIG61, NISBD2, mENA, HER1, ERBB1, ERBB, epidermal growth factor receptor
Anti-EGFR Antibody (6P389) is a Rabbit antibody targeting EGFR. Anti-EGFR Antibody (6P389) can be used in ELISA,ELISA(Det),ICC/IF.
Anti-EGFR Antibody (6P389)
Anti-EGFR Antibody (6P389)
😃Good
Catalog No. TMAY-00024Alias PIG61, NISBD2, mENA, HER1, ERBB1, ERBB, epidermal growth factor receptor
Anti-EGFR Antibody (6P389) is a Rabbit antibody targeting EGFR. Anti-EGFR Antibody (6P389) can be used in ELISA,ELISA(Det),ICC/IF.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 20 μL | $94 | 7-10 days | |
| 100 μL | $269 | 7-10 days |
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Product Introduction
Bioactivity
Related Conjugates and Formulations
Antigen Details
Chemical Properties
| Description | Antibody Type: Rabbit Monoclonal Application: ELISA, ELISA(Det), ICC/IF Reactivity: Human |
| Alias | PIG61, NISBD2, mENA, HER1, ERBB1, ERBB, epidermal growth factor receptor |
| Ig Type | Rabbit IgG |
| Clone | 6P389 |
| Reactivity | Human |
| Verified Activity | Immunofluorescence staining of Human EGFR in A431 cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-Human EGFR monoclonal antibody (1:300) at 4°C overnight. Then cells were stained with the Alexa Fluor® 488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue). |
| Application | ELISA,ELISA(Det),ICC/IF |
| Recommended Dose | ELISA: 1:25000-1:50000; ICC-IF: 1:100-1:500; ELISA(Det): 1:5000-1:50000 |
| Antibody Type | Monoclonal |
| Host Species | Rabbit |
| Construction | This antibody was obtained from a rabbit immunized with purified, recombinant Human EGFR (rh EGFR; TMPY-00742; Met 1-Ser 645; NP_005219) . |
| Purification | Protein A |
| Appearance | Liquid |
| Formulation | 0.2 μm filtered solution in PBS |
| Research Background | As a member of the epidermal growth factor receptor (EGFR) family, EGFR protein is type I transmembrane glycoprotein that binds a subset of EGF family ligands including EGF, amphiregulin, TGF-α, betacellulin, etc. EGFR protein plays a crucial role in signaling pathway in the regulation of cell proliferation, survival and differentiation. Binding of a ligand induces EGFR protein homo- or heterodimerization, the subsequent tyrosine autophosphorylation and initiates various down stream pathways (MAPK, PI3K/PKB and STAT). In addition, EGFR signaling also has been shown to exert action on carcinogenesis and disease progression, and thus EGFR protein is proposed as a target for cancer therapy currently.Cancer ImmunotherapyImmune CheckpointImmunotherapyTargeted Therapy |
| Conjucates | Unconjugated |
| Others Formats | HRP |
| Antibody Types Available | 2 |
| Immunogen | Recombinant Protein: Human EGFR Protein（Catalog#10001-H08H） |
| Antigen Species | Human |
| Biology Area | Cancer Drug Targets, Receptor Tyrosine Kinases (RTKs) |
Stability & Storage
| Stability & Storage | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free. |
| Transport | Shipping with blue ice. |
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For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . 
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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