HER3/ERBB3 Protein, Human, Recombinant (His), Biotinylated

ErbB3, also known as Her3(human epidermal growth factor receptor3), is a member of the epidermal growth factor receptor (EGFR) family of receptor tyrosine kinases. This membrane-bound glycoprotein has a neuregulin binding domain but has not an active kinase domain., and therefore can not mediate the intracellular signal transduction through protein phosphorylation. However, its heterodimer with ErbB2 or other EGFR members responsible for tyrosine phosphorylation forms a receptor complex with high affinity, and initiates the related pathway which lead to cell proliferation or differentiation. ErbB3 has been shown to implicated in numerous cancers, including prostate, bladder, and breast tumors. This protein has different isoforms derived from alternative splicing variants, and among which, the secreted isoform lacking the intermembrane region modulates the activity of membrane-bound form.Cancer ImmunotherapyImmune CheckpointImmunotherapyTargeted Therapy

Pack Size	Availability	Price/USD
20 μg	In stock	$ 256.00
100 μg	5 days	$ 589.00
200 μg	5 days	$ 1,000.00
500 μg	5 days	$ 2,030.00

Biological Information

Immobilized Recombinant Human NRG1 / Neuregulin 1 Protein (Fc Tag) at 2 μg/mL (100 μL/well) can bind Recombinant Human HER3 / ErbB3 Protein (His Tag), Biotinylated , the EC50 is 100-300 ng/mL.

Description

Species	Human
Expression System	HEK293
Tag	His
Accession Number	P21860-1
Synonyms	MDA-BF-1, c-erbB-3, HER3, p85-sErbB3, LCCS2, p180-ErbB3, erbB3-S, erb-b2 receptor tyrosine kinase 3, EEBB3, p45-sErbB3, ErbB-3, c-erbB3
Construction	A DNA sequence encoding the extracellular domain (Met1-Thr643) of human ErbB3 (NP_001973.2) precursor was expressed with a C-terminal polyhistidine tag. The purified protein was biotinylated in vitro.
Protein Purity	> 95% as determined by SDS-PAGE.
Molecular Weight	Approxiamtely 70.2 kDa

Endotoxin	< 1.0 EU per μg protein as determined by the LAL method.
Formulation	Lyophilized from sterile PBS,pH7.4. Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0. 01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
Reconstitution	A hardcopy of datasheet with reconstitution instructions is sent along with the products. Please refer to it for detailed information.
Stability & Storage	Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Shipping	In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Research Background	ErbB3, also known as Her3(human epidermal growth factor receptor3), is a member of the epidermal growth factor receptor (EGFR) family of receptor tyrosine kinases. This membrane-bound glycoprotein has a neuregulin binding domain but has not an active kinase domain., and therefore can not mediate the intracellular signal transduction through protein phosphorylation. However, its heterodimer with ErbB2 or other EGFR members responsible for tyrosine phosphorylation forms a receptor complex with high affinity, and initiates the related pathway which lead to cell proliferation or differentiation. ErbB3 has been shown to implicated in numerous cancers, including prostate, bladder, and breast tumors. This protein has different isoforms derived from alternative splicing variants, and among which, the secreted isoform lacking the intermembrane region modulates the activity of membrane-bound form.Cancer ImmunotherapyImmune CheckpointImmunotherapyTargeted Therapy