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Synonyms: serpin peptidase inhibitor, clade F,member 1, Sdf3, Pedfl, Pedf, EPC-1, AI195227

| Pack Size | Price | USA Stock | Global Stock | Quantity |
|---|---|---|---|---|
| 5 μg | $87 | 7-10 days | 7-10 days | |
| 10 μg | $142 | 7-10 days | 7-10 days | |
| 20 μg | $233 | 7-10 days | 7-10 days | |
| 50 μg | $535 | 7-10 days | 7-10 days |
| Bioactivity | Measured by its binding ability in a functional ELISA. Immobilized mouse SERPINF1-His at 10 μg/ml (100 μl/well) can bind biotinylated human GST-CSNK2A1 with a linear range of 0.31-2.5 μg/ml. |
| Description | PEDF Protein, Mouse, Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 45.8 kDa and the accession number is P97298. |
| Species | Mouse |
| Expression System | HEK293 Cells |
| Tag | C-His |
| Accession Number | P97298 |
| Construction | A DNA sequence encoding the extracellular domain (Met 1-Thr 417) of mouse SerpinF1 (NP_035470.3) precursor was expressed with a C-terminal polyhistidine tag. Predicted N terminal: Gln 20 |
| Protein Purity | > 95 % as determined by SDS-PAGE |
| Endotoxin | < 1.0 EU/μg of the protein as determined by the LAL method. |
| Formulation | Lyophilized from a solution filtered through a 0.22 μm filter, containing 25 mM MES, 150 mM Nacl, 0.02% Tween, 20, 5% Trehalose, pH 5. 5. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization. |
| Reconstitution | A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information. |
| Stability & Storage | It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots. |
| Shipping | In general, lyophilized powders are shipped with blue ice, while solutions are shipped with dry ice. |
| Synonyms | serpin peptidase inhibitor, clade F,member 1, Sdf3, Pedfl, Pedf, EPC-1, AI195227 |
| Research Background |
| Molecular Weight | 45.8 kDa (predicted); 60-65 kDa (reducing condition, due to glycosylation) |
| Storage | Lyophilized powder: -20~-80°C for 1 year | Solution: -80°C for 6 months |
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