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Rabbit Anti-Mouse IgM Secondary Antibody (6Z948)
😃Good
Catalog No. TMAY-00009
Alias muH, Igm, Igh-M, Ighm, Igh-6, Igh6
Rabbit Anti-Mouse IgM Secondary Antibody (6Z948) is a Rabbit antibody targeting IgM. Rabbit Anti-Mouse IgM Secondary Antibody (6Z948) can be used in ELISA.
Rabbit Anti-Mouse IgM Secondary Antibody (6Z948)
Rabbit Anti-Mouse IgM Secondary Antibody (6Z948)
😃Good
Catalog No. TMAY-00009Alias muH, Igm, Igh-M, Ighm, Igh-6, Igh6
Rabbit Anti-Mouse IgM Secondary Antibody (6Z948) is a Rabbit antibody targeting IgM. Rabbit Anti-Mouse IgM Secondary Antibody (6Z948) can be used in ELISA.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 100 μL | $199 | 7-10 days |
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Chemical Properties
| Description | Rabbit Anti-Mouse IgM Secondary Antibody (6Z948) is a Rabbit antibody targeting IgM. Rabbit Anti-Mouse IgM Secondary Antibody (6Z948) can be used in ELISA. |
| Alias | muH, Igm, Igh-M, Ighm, Igh-6, Igh6 |
| Ig Type | Monoclonal Rabbit IgG |
| Clone | 6Z948 |
| Reactivity | Mouse |
| Verified Activity | ELISA of immunoglobulins shows horseradish-peroxidase (HRP) conjugated Recombinant Anti-IgM Antibody, Rabbit Monoclonal reacts to mouse IgM. No cross reactivity with mouse IgG1, mouse IgG2a, mouse IgG2b, mouse IgG3, mouse IgA, human IgG. The plate was coated with 1 μg/mL of different immunoglobulins. 0.4 μg/mL of horseradish-peroxidase (HRP) conjugated Recombinant Anti-IgM Antibody, Rabbit Monoclonal was used as the secondary antibody. (Validation Experiment) |
| Application | ELISA |
| Recommended Dose | ELISA: 1:5000-1:10000 |
| Antibody Type | Monoclonal |
| Host Species | Rabbit |
| Construction | This antibody was obtained from a rabbit immunized with purified mouse IgM protein. |
| Purification | Protein A |
| Appearance | Liquid |
| Formulation | 0.2 μm filtered solution in 20 mM MES, 100 mM NaCl, pH 6.0 |
| Research Background | Antibodies, also known as Immunoglobulins ( Ig ), are gamma globulin proteins that are found in blood or other bodily fluids of vertebrates and are used by the immune system to identify and neutralize foreign objects, such as bacteria and viruses. Antibodies can come in different varieties known as isotypes or classes. In placental mammals, there are five antibody isotypes known as IgA, IgD, IgE, IgG, and IgM. They are each named with an " Ig " prefix that stands for immunoglobulin, another name for antibody, and differ in their biological properties, functional locations, and ability to deal with different antigens. IgM is expressed on the surface of B cells and in a secreted form with very high avidity. It eliminates pathogens in the early stages of B cell-mediated immunity before there is sufficient IgG. IgM is by far the physically largest antibody in the human circulatory system. It is produced after an animal has been exposed to an antigen for an extended time or when an animal is exposed to an antigen for the second time. IgM forms polymers where multiple immunoglobulins are covalently linked together with disulfide bonds, mostly as a pentamer but also as a hexamer. Because each monomer has two antigen-binding sites, a pentameric IgM has 1 binding site. Typically, however, IgM cannot bind 1 antigen at the same time because the large size of most antigens hinders binding to nearby sites. Due to its polymeric nature, IgM possesses high avidity and is particularly effective at complement activation. |
| Conjucates | Unconjugated |
| Others Formats | Biotin/HRP |
| Antibody Types Available | 3 |
| Immunogen | Purified Mouse IgM Protein |
| Antigen Species | Mouse |
Stability & Storage
| Stability & Storage | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free. |
| Transport | Shipping with blue ice. |
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For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . 
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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