Anti-CD81 Antibody (2M358) is a Mouse antibody targeting CD81. Anti-CD81 Antibody (2M358) can be used in WB,FCM,ELISA.

IgG1

2M358

Human

1. Blank control (black line): HepG2.
Primary Antibody (green line): Mouse Anti-CD81 antibody (TMAB-04077)
Dilution: 1 μg/Test;
Secondary Antibody (white blue line): Goat anti-mouse IgG-FITC
Dilution: 0.5 μg/Test.
Isotype control (orange line): Normal Mouse IgG
Protocol
The cells were incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
2. 25 μg total protein per lane of various lysates (see on figure) probed with CD81 monoclonal antibody, unconjugated (TMAB-04077) at 1:5000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r. T. for 60 min.

WB: 1:500-5000; FCM: 1μg/Test; ELISA: 1:5000-10000

Monoclonal

KLH conjugated synthetic peptide: human CD81

Human

CD81

CD81 antigen

May play an important role in the regulation of lymphoma cell growth. Interacts with a 16-kDa Leu-13 protein to form a complex possibly involved in signal transduction. May acts a the viral receptor for HCV.