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TNF alpha Protein, Human, Recombinant (His & Avi)
(Synonyms: TNF-α, TNFα, TNFSF2, TNFSF1A, TNFATNF, TNF-alpha, TNFalpha, TNFA, TNF α, TNF, DIF, Cachectin, APC1 protein) Copy Product InfoSynonyms: TNF-α, TNFα, TNFSF2, TNFSF1A, TNFATNF, TNF-alpha, TNFalpha, TNFA, TNF α, TNF, DIF, Cachectin, APC1 protein
Catalog No. TMPK-00120 Copy Product Info
TNF alpha Protein, Human, Recombinant (His & Avi) is expressed in HEK293 mammalian cells with C-His-Avi tag. The predicted molecular weight is 20.3 kDa and the accession number is P01375.
| Pack Size | Price | USA Stock | Global Stock | Quantity |
|---|---|---|---|---|
| 5 μg | $44 | - | In Stock | |
| 10 μg | $68 | - | In Stock | |
| 20 μg | $108 | 7-10 days | 7-10 days | |
| 50 μg | $198 | 7-10 days | 7-10 days | |
| 100 μg | $347 | - | In Stock | |
| 200 μg | $628 | 7-10 days | 7-10 days | |
| 500 μg | $1,400 | 7-10 days | 7-10 days |
For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)
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For research use only—not for human use. No sales to individuals. Use as intended only.
Batch Information
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Purity:> 90% as determined by Bis-Tris PAGE > 95% as determined by HPLC
Appearance:Lyophilized powder
Color:White
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Product Introduction
Bioactivity
Chemical Properties
Storage & Solubility Information
| Bioactivity | 1. Immobilized Human TNF alpha, His Tag at 5μg/ml (100μl/well) on the plate. Dose response curve for Human TNFR2, mFc Tag with the EC50 of 61.0ng/ml determined by ELISA.
2. Human TNF alpha, His Tag captured on CM5 Chip via anti-his antibody can bind Human TNFR2, mFc Tag with an affinity constant of 1.62 nM as determined in SPR assay. |
| Description | TNF alpha Protein, Human, Recombinant (His & Avi) is expressed in HEK293 mammalian cells with C-His-Avi tag. The predicted molecular weight is 20.3 kDa and the accession number is P01375. |
| Species | Human |
| Expression System | HEK293 Cells |
| Tag | C-His-Avi |
| Accession Number | P01375 |
| Construction | Val77-Leu233 |
| Protein Purity | > 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC |
| Endotoxin | < 1.0 EU/μg of the protein as determined by the LAL method. |
| Formulation | Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS (pH 7.4). Typically, 8% trehalose is incorporated as a protective agent before lyophilization. |
| Reconstitution | Reconstitute the lyophilized protein in distilled water. The product concentration should not be less than 100 μg/ml. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing. |
| Stability & Storage | It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots. |
| Shipping | In general, lyophilized powders are shipped with blue ice, while solutions are shipped with dry ice. |
| Synonyms | TNF-α, TNFα, TNFSF2, TNFSF1A, TNFATNF, TNF-alpha, TNFalpha, TNFA, TNF α, TNF, DIF, Cachectin, APC1 protein |
| Research Background |
| Molecular Weight | 20.3 kDa (predicted). Due to glycosylation, the protein migrates to 21-25 kDa based on Tris-Bis PAGE result. |
| Storage | Store at low temperature Lyophilized powder: -20~-80°C for 1 year | Solution: -80°C for 6 months |
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Dose Conversion
You can also refer to dose conversion for different animals. More Dose Conversion
Tech Support
Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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Related Tags: TNF alpha Protein, Human, Recombinant (His & Avi) chemical structure | TNF alpha Protein, Human, Recombinant (His & Avi) molecular weight
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