Anti-HES1 Polyclonal Antibody 3 is a Rabbit antibody targeting HES1. Anti-HES1 Polyclonal Antibody 3 can be used in WB,ELISA,ICC/IF.

IgG

Human,Rat (predicted:Mouse)

1. Measured by its binding ability in a indirect ELISA. Immobilized Human HES1 protein, His Tag at 2 μg/mL (100 μL/well) can bind  Rabbit Anti-Human HES1 Antibody, the EC50 is 178.8 ng/mL.
2. Sample:
Lane 1: Rat Kidney tissue lysates
Lane 2: Rat Liver tissue lysates
Primary: Anti-HES1 (TMAB-06988) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 30 kDa
Observed band size: 35 kDa
3. Sample:
Lane 1: Recombinant human HES1 protein
Primary: Anti-HES1 (TMAB-06988) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 30 kDa
Observed band size: 30 kDa
4. 4% Paraformaldehyde-fixed MCF-7 (H) cell; Triton X-100 at r. T. for 20 min; Antibody incubation with (HES1) polyclonal Antibody, unconjugated (TMAB-06988) 1:100, 90 min at 37°C; followed by conjugated Goat Anti-Rabbit IgG antibody (green) at 37°C for 90 min, DAPI (blue) was used to stain the cell nuclei. PBS instead of the primary antibody was used as the blank control.

WB: 1:500-2000; ELISA: 1:5000-10000; ICC/IF: 1:50-200

Polyclonal

Recombinant Protein: human HES1 protein

Human

HES1

Transcription factor HES-1

Transcriptional repressor of genes that require a bHLH protein for their transcription. May act as a negative regulator of myogenesis by inhibiting the functions of MYOD1 and ASH1. Binds DNA on N-box motifs: 5'-CACNAG-3' with high affinity and on E-box motifs: 5'-CANNTG-3' with low affinity.