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Anti-PMVK Antibody (7Y287)
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Catalog No. TMAY-02762
Alias PMKASE, PMKA, PMK, phosphomevalonate kinase, HUMPMKI
Anti-PMVK Antibody (7Y287) is a Rabbit antibody targeting PMVK. Anti-PMVK Antibody (7Y287) can be used in WB,ELISA,IP.
Anti-PMVK Antibody (7Y287)
Anti-PMVK Antibody (7Y287)
😃Good
Catalog No. TMAY-02762Alias PMKASE, PMKA, PMK, phosphomevalonate kinase, HUMPMKI
Anti-PMVK Antibody (7Y287) is a Rabbit antibody targeting PMVK. Anti-PMVK Antibody (7Y287) can be used in WB,ELISA,IP.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 100 μL | $268 | 7-10 days |
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Product Introduction
Bioactivity
Related Conjugates and Formulations
Antigen Details
Chemical Properties
| Description | Anti-PMVK Antibody (7Y287) is a Rabbit antibody targeting PMVK. Anti-PMVK Antibody (7Y287) can be used in WB,ELISA,IP. |
| Alias | PMKASE, PMKA, PMK, phosphomevalonate kinase, HUMPMKI |
| Ig Type | Monoclonal Rabbit IgG |
| Clone | 7Y287 |
| Reactivity | Human |
| Verified Activity | 1. Anti-PMVK rabbit monoclonal antibody at 1:500 dilution. -Lane A: HepG2 Whole Cell lysate. -Lysates/proteins at 30 μg per lane. -Secondary -Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution. -Developed using the Odyssey technique. -Performed under reducing conditions. -Predicted band size:21 kDa. -Observed band size:24 kDa(We are unsure as to the identity of these extra bands.) 2. PMVK was immunoprecipitated using: -Lane A:0.5 mg Hela Whole Cell Lysate. -Lane B:0.5 mg NIH-3T3 Whole Cell Lysate -0.5 µL anti-PMVK rabbit monoclonal antibody and 15 μl of 50 % Protein G agarose. -Primary antibody: -Anti-PMVK rabbit monoclonal antibody, at 1:500 dilution. -Secondary antibody: -Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution. -Developed using the odyssey technique. -Performed under reducing conditions. -Predicted band size: 21 kDa. -Observed band size: 21 kDa |
| Application | WB,ELISA,IP |
| Recommended Dose | WB: 1:500-1:1000; ELISA: 1:25000-1:50000; IP: 0.2-1 μL/mg of lysate |
| Antibody Type | Monoclonal |
| Host Species | Rabbit |
| Construction | This antibody was obtained from a rabbit immunized with purified, recombinant Human PMVK / phosphomevalonate kinase (rh PMVK / phosphomevalonate kinase; TMPY-04767; Q15126; Met1-Leu192). |
| Purification | Protein A |
| Appearance | Liquid |
| Formulation | 0.2 μm filtered solution in PBS |
| Research Background | PMVK is a peroxisomal enzyme that catalyzes the conversion of mevalonate 5-phosphate into mevalonate 5-diphosphate, the fifth reaction of the cholesterol biosynthetic pathway. Studies in rat show that the message level and the enzyme activity of PMVK is regulated by sterol, and that this regulation is coordinated with 3-hydroxy-3-methylglutaryl coenzyme A reductase, the rate-limiting enzyme of cholesterol biosynthesis. |
| Conjucates | Unconjugated |
| Immunogen | Recombinant Human PMVK / phosphomevalonate kinase Protein (TMPY-04767) |
| Antigen Species | Human |
Stability & Storage
| Stability & Storage | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free. |
| Transport | Shipping with blue ice. |
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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