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Anti-NUP153 Antibody (8V777) is an antibody targeting NUP153. Anti-NUP153 Antibody (8V777) can be used in ELISA, IHC, IF.
Pack Size | Price | Availability | Quantity |
---|---|---|---|
50 μL | $209 | 7-10 days | |
100 μL | $348 | 7-10 days |
Description | Antibody Type: Recombinant Rabbit Monoclonal Application: ELISA, IHC, IF Reactivity: Human |
Alias | NUP 153, Nucleoporin Nup153, Nuclear pore complex protein Nup153, 153 kDa nucleoporin |
Ig Type | Rabbit IgG |
Clone | 8V777 |
Reactivity | Human |
Verified Activity | 1. IHC image of TMAH-00839 diluted at 1:100 and staining in paraffin-embedded human breast cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB. 2. Immunofluorescence staining of Hela Cells with TMAH-00839 at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeated by 0.2% TritonX-100, and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L). |
Application | ELISA, IHC, IF |
Recommended Dose | IHC:1:50-1:200; IF:1:20-1:200. |
Antibody Type | Monoclonal |
Subcellular Localization | Nucleus. Nucleus membrane. Nucleus, nuclear pore complex. |
Construction | Recombinant Antibody |
Purification | Affinity-chromatography |
Appearance | Liquid |
Formulation | Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
Research Background | Component of the nuclear pore complex (NPC), a complex required for the trafficking across the nuclear envelope. Functions as a scaffolding element in the nuclear phase of the NPC essential for normal nucleocytoplasmic transport of proteins and mRNAs. Involved in the quality control and retention of unspliced mRNAs in the nucleus; in association with TPR, regulates the nuclear export of unspliced mRNA species bearing constitutive transport element (CTE) in a NXF1- and KHDRBS1-independent manner. Mediates TPR anchoring to the nuclear membrane at NPC. The repeat-containing domain may be involved in anchoring other components of the NPC to the pore membrane. Possible DNA-binding subunit of the nuclear pore complex (NPC). (Microbial infection) Binds HIV-1 capsid-nucleocapsid (HIV-1 CA-NC) complexes and thereby promotes the integration of the virus in the nucleus of non-dividing cells (in vitro). (Microbial infection) Binds HIV-2 protein vpx and thereby promotes the nuclear translocation of the lentiviral genome (in vitro). |
Conjucates | Unconjugated |
Immunogen | A synthetic peptide: Human Nup153 |
Antigen Species | Human |
Gene ID | 9972 |
Uniprot ID | |
Biology Area | Epigenetics and Nuclear Signaling, Tags & Cell Markers, Signal transduction |
Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
Transport | Shipping with blue ice. |
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