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Anti-NUP153 Antibody (8V777)

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Catalog No. TMAH-00839
Alias NUP 153, Nucleoporin Nup153, Nuclear pore complex protein Nup153, 153 kDa nucleoporin

Anti-NUP153 Antibody (8V777) is an antibody targeting NUP153. Anti-NUP153 Antibody (8V777) can be used in ELISA, IHC, IF.

Anti-NUP153 Antibody (8V777)

Anti-NUP153 Antibody (8V777)

😃Good
Catalog No. TMAH-00839Alias NUP 153, Nucleoporin Nup153, Nuclear pore complex protein Nup153, 153 kDa nucleoporin
Anti-NUP153 Antibody (8V777) is an antibody targeting NUP153. Anti-NUP153 Antibody (8V777) can be used in ELISA, IHC, IF.
Pack SizePriceAvailabilityQuantity
50 μL$209 7-10 days
100 μL$348 7-10 days
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Product Introduction

Bioactivity
Description
Antibody Type: Recombinant Rabbit Monoclonal

Application: ELISA, IHC, IF

Reactivity: Human
AliasNUP 153, Nucleoporin Nup153, Nuclear pore complex protein Nup153, 153 kDa nucleoporin
Ig Type
Rabbit IgG
Clone
8V777
Reactivity
Human
Verified Activity
1. IHC image of TMAH-00839 diluted at 1:100 and staining in paraffin-embedded human breast cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
2. Immunofluorescence staining of Hela Cells with TMAH-00839 at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeated by 0.2% TritonX-100, and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L).
Application
ELISA, IHC, IF
Recommended Dose
IHC:1:50-1:200; IF:1:20-1:200.
Antibody Type
Monoclonal
Subcellular LocalizationNucleus. Nucleus membrane. Nucleus, nuclear pore complex.
ConstructionRecombinant Antibody
PurificationAffinity-chromatography
AppearanceLiquid
FormulationPhosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Research BackgroundComponent of the nuclear pore complex (NPC), a complex required for the trafficking across the nuclear envelope. Functions as a scaffolding element in the nuclear phase of the NPC essential for normal nucleocytoplasmic transport of proteins and mRNAs. Involved in the quality control and retention of unspliced mRNAs in the nucleus; in association with TPR, regulates the nuclear export of unspliced mRNA species bearing constitutive transport element (CTE) in a NXF1- and KHDRBS1-independent manner. Mediates TPR anchoring to the nuclear membrane at NPC. The repeat-containing domain may be involved in anchoring other components of the NPC to the pore membrane. Possible DNA-binding subunit of the nuclear pore complex (NPC). (Microbial infection) Binds HIV-1 capsid-nucleocapsid (HIV-1 CA-NC) complexes and thereby promotes the integration of the virus in the nucleus of non-dividing cells (in vitro). (Microbial infection) Binds HIV-2 protein vpx and thereby promotes the nuclear translocation of the lentiviral genome (in vitro).
Related Conjugates and Formulations
Conjucates
Unconjugated
Antigen Details
Immunogen
A synthetic peptide: Human Nup153
Antigen Species
Human
Gene ID
9972
Uniprot ID
Biology Area
Epigenetics and Nuclear Signaling, Tags & Cell Markers, Signal transduction
Chemical Properties
Stability & Storage
Stability & StorageStore at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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