Antibody Type: Rabbit Monoclonal

Application: WB, ELISA, ICC/IF, IP

Reactivity: Human

Rabbit IgG

4I293

Human

1. Anti-AKR1B1 rabbit monoclonal antibody at 1:500 dilution.
-Lane A: Jurkat Whole Cell Lysate.
-Lane B: Hela Whole Cell Lysate.
-Lane C: A431 Whole Cell lysate.
-Lysates/proteins at 30 μg per lane.
-Secondary
-Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.
-Developed using the Odyssey technique.
-Performed under reducing conditions.
-Predicted band size:35 kDa.
-Observed band size:37 kDa.
2. AKR1B1 was immunoprecipitated using:
-Lane A:0.5 mg Jurkat Whole Cell Lysate
-0.5 µL anti-AKR1B1 rabbit monoclonal antibody and 15 μl of 50 % Protein G agarose.
-Primary antibody:
-Anti-AKR1B1 rabbit monoclonal antibody, at 1:500 dilution.
-Secondary antibody:
-Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution.
-Developed using the odyssey technique.
-Performed under reducing conditions.
-Predicted band size: 35 kDa.
-Observed band size: 35 kDa.
3. Immunofluorescence staining of Human AKR1B1 in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-Human AKR1B1 monoclonal antibody (dilution ratio 1:60) at 4°C overnight. Then cells were stained with the Alexa Fluor® 488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to cytoplasm and nucleus.

WB,ELISA,ICC/IF,IP

WB: 1:500-1:2000; ELISA: 1:5000-1:10000; ICC-IF: 1:20-1:100; IP: 0.2-1 μL/mg of lysate

Monoclonal

Unconjugated

Recombinant Protein: Human AKR1B1 protein (TMPY-02438)

Human