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Anti-AKR1B1 Antibody (4I293)

😃Good
Catalog No. TMAY-01832
Alias MGC1804, AR, ALR2, ALDR1, aldo-keto reductase family 1, member B1 (aldose reductase), ADR

Anti-AKR1B1 Antibody (4I293) is a Rabbit antibody targeting AKR1B1. Anti-AKR1B1 Antibody (4I293) can be used in WB,ELISA,ICC/IF,IP.

Anti-AKR1B1 Antibody (4I293)

Anti-AKR1B1 Antibody (4I293)

😃Good
Catalog No. TMAY-01832Alias MGC1804, AR, ALR2, ALDR1, aldo-keto reductase family 1, member B1 (aldose reductase), ADR
Anti-AKR1B1 Antibody (4I293) is a Rabbit antibody targeting AKR1B1. Anti-AKR1B1 Antibody (4I293) can be used in WB,ELISA,ICC/IF,IP.
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100 μL$270 7-10 days
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Product Introduction

Bioactivity
Description
Anti-AKR1B1 Antibody (4I293) is a Rabbit antibody targeting AKR1B1. Anti-AKR1B1 Antibody (4I293) can be used in WB,ELISA,ICC/IF,IP.
AliasMGC1804, AR, ALR2, ALDR1, aldo-keto reductase family 1, member B1 (aldose reductase), ADR
Ig Type
Monoclonal Rabbit IgG
Clone
4I293
Reactivity
Human
Verified Activity
1. Anti-AKR1B1 rabbit monoclonal antibody at 1:500 dilution.

-Lane A: Jurkat Whole Cell Lysate.

-Lane B: Hela Whole Cell Lysate.

-Lane C: A431 Whole Cell lysate.

-Lysates/proteins at 30 μg per lane.

-Secondary

-Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.

-Developed using the Odyssey technique.

-Performed under reducing conditions.

-Predicted band size:35 kDa.

-Observed band size:37 kDa.

2. AKR1B1 was immunoprecipitated using:

-Lane A:0.5 mg Jurkat Whole Cell Lysate

-0.5 µL anti-AKR1B1 rabbit monoclonal antibody and 15 μl of 50 % Protein G agarose.

-Primary antibody:

-Anti-AKR1B1 rabbit monoclonal antibody, at 1:500 dilution.

-Secondary antibody:

-Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution.

-Developed using the odyssey technique.

-Performed under reducing conditions.

-Predicted band size: 35 kDa.

-Observed band size: 35 kDa.

3. Immunofluorescence staining of Human AKR1B1 in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-Human AKR1B1 monoclonal antibody (dilution ratio 1:60) at 4°C overnight. Then cells were stained with the Alexa Fluor® 488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to cytoplasm and nucleus.
Application
WB,ELISA,ICC/IF,IP
Recommended Dose
WB: 1:500-1:2000; ELISA: 1:5000-1:10000; ICC-IF: 1:20-1:100; IP: 0.2-1 μL/mg of lysate
Antibody Type
Monoclonal
Host SpeciesRabbit
ConstructionThis antibody was obtained from a rabbit immunized with purified, recombinant Human AKR1B1 (rh AKR1B1; TMPY-02438; P15121; Met1-Phe316).
PurificationProtein A
AppearanceLiquid
Formulation0.2 μm filtered solution in PBS
Research BackgroundAldose reductase (AKR1B1) belongs to the aldo/keto reductase superfamily. AKR1B1 is a NADPH-dependent aldo-keto reductase best known as the rate-limiting enzyme of the polyol pathway. Expression of AKR1B1 was the highest in lens and retina. It is the first enzyme in the polyol pathway through which glucose is converted to sorbitol which is important for the function of various organs in the body, and has been implicated in the etiology of diabetic complications. AKR1B1 is quite abundant in the collecting tubule cells and thought to provide protection against hypertonic environment. Some human tissues contain AKR1B1 as well as AKR1B10, a closely related member of the aldo-keto reductase superfamily.
Related Conjugates and Formulations
Conjucates
Unconjugated
Antigen Details
Immunogen
Recombinant Human AKR1B1 protein (TMPY-02438)
Antigen Species
Human
Chemical Properties
Stability & Storage
Stability & StorageStore at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free.
TransportShipping with blue ice.

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Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc

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