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Anti-ITGB4 Antibody (5T264)
😃Good
Catalog No. TMAH-00651
Anti-ITGB4 Antibody (5T264) is an antibody targeting ITGB4. Anti-ITGB4 Antibody (5T264) can be used in ELISA, FC.
Anti-ITGB4 Antibody (5T264)
Anti-ITGB4 Antibody (5T264)
😃Good
Catalog No. TMAH-00651
Anti-ITGB4 Antibody (5T264) is an antibody targeting ITGB4. Anti-ITGB4 Antibody (5T264) can be used in ELISA, FC.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 50 μL | $208 | 7-10 days | |
| 100 μL | $349 | 7-10 days |
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Product Introduction
Bioactivity
Related Conjugates and Formulations
Antigen Details
Chemical Properties
| Description | Antibody Type: Recombinant Monoclonal Application: ELISA, FC Reactivity: Human |
| Ig Type | Mouse IgG2a |
| Clone | 5T264 |
| Reactivity | Human |
| Verified Activity | Overlay Peak curve showing A549 cells surface stained with TMAH-00651 (red line) at 1:100. Then 10% normal goat serum was Incubated to block non-specific protein-protein interactions followed by the antibody (1µg/1*10^6 cells) for 45 min at 4°C. The secondary antibody used was FITC-conjugated Goat Anti-Mouse IgG(H+L) at 1/200 dilution for 35 min at 4°C. Isotype control antibody (green line) was mouse IgG1 (1µg/1*10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed. |
| Application | ELISA, FC |
| Recommended Dose | FC:1:20-1:500. |
| Antibody Type | Monoclonal |
| Subcellular Localization | Cell membrane; Single-pass type I membrane protein. Cell membrane; Lipid-anchor. Cell junction, hemidesmosome. Note=Colocalizes with DST at the leading edge of migrating keratinocytes. |
| Construction | Recombinant Antibody |
| Purification | Affinity-chromatography |
| Appearance | Liquid |
| Formulation | Preservative: 0.03% Proclin 300. Constituents: 50% Glycerol, 0.01M PBS, PH 7.4. |
| Research Background | Integrin alpha-6/beta-4 is a receptor for laminin. Plays a critical structural role in the hemidesmosome of epithelial cells. Is required for the regulation of keratinocyte polarity and motility. ITGA6:ITGB4 binds to NRG1 (via EGF domain) and this binding is essential for NRG1-ERBB signaling. ITGA6:ITGB4 binds to IGF1 and this binding is essential for IGF1 signaling. ITGA6:ITGB4 binds to IGF2 and this binding is essential for IGF2 signaling. |
| Conjucates | Unconjugated |
| Immunogen | Recombinant Protein: Human ITGB4 Protein |
| Antigen Species | Human |
| Gene ID | 3691 |
| Uniprot ID | |
| Biology Area | Signal transduction |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . 
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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