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Anti-Phospho-ATF2 (Thr71) Antibody (5C542)
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Catalog No. TMAH-00882
Alias TREB7, Phospho-ATF2 (T71), p-ATF2 (Thr71), p-ATF2 (T71), HB16, CRE-BP1, CREB-2, CREB2, ATF2 (p-Thr71), ATF2 (p-T71), activating transcription factor 2
Anti-Phospho-ATF2 (Thr71) Antibody (5C542) is an antibody targeting Phospho-ATF2 (Thr71). Anti-Phospho-ATF2 (Thr71) Antibody (5C542) can be used in ELISA, WB, IF.
Anti-Phospho-ATF2 (Thr71) Antibody (5C542)
Anti-Phospho-ATF2 (Thr71) Antibody (5C542)
😃Good
Catalog No. TMAH-00882Alias TREB7, Phospho-ATF2 (T71), p-ATF2 (Thr71), p-ATF2 (T71), HB16, CRE-BP1, CREB-2, CREB2, ATF2 (p-Thr71), ATF2 (p-T71), activating transcription factor 2
Anti-Phospho-ATF2 (Thr71) Antibody (5C542) is an antibody targeting Phospho-ATF2 (Thr71). Anti-Phospho-ATF2 (Thr71) Antibody (5C542) can be used in ELISA, WB, IF.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 50 μL | $209 | 7-10 days | |
| 100 μL | $349 | 7-10 days |
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Product Introduction
Bioactivity
Related Conjugates and Formulations
Antigen Details
Chemical Properties
| Description | Antibody Type: Recombinant Monoclonal Application: ELISA, WB, IF Reactivity: Human |
| Alias | TREB7, Phospho-ATF2 (T71), p-ATF2 (Thr71), p-ATF2 (T71), HB16, CRE-BP1, CREB-2, CREB2, ATF2 (p-Thr71), ATF2 (p-T71), activating transcription factor 2 |
| Ig Type | Rabbit IgG |
| Clone | 5C542 |
| Reactivity | Human |
| Verified Activity | 1. Western Blot -Positive WB detected in 293 whole cell lysate,A549 whole cell lysate,HepG2 whole cell lysate(treated with Calyculin A or EGF) -All lanes Phospho-ATF2 antibody at 1.015μg/ml -Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution -Predicted band size: 70 KDa -Observed band size: 70 KDa 2. Immunofluorescence staining of A549 cells(treated with 100mM EGF for 20min) with TMAH-00882 at 1:63,counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L). |
| Application | ELISA, WB, IF |
| Recommended Dose | WB:1:500-1:5000; IF:1:20-1:200. |
| Antibody Type | Monoclonal |
| Subcellular Localization | Nucleus. Cytoplasm. Mitochondrion outer membrane. Note=Shuttles between the cytoplasm and the nucleus and heterodimerization with JUN is essential for the nuclear localization. Localization to the cytoplasm is observed under conditions of cellular stress and in disease states. Localizes at the mitochondrial outer membrane in response to genotoxic stress. Phosphorylation at Thr-52 is required for its nuclear localization and negatively regulates its mitochondrial localization. Co-localizes with the MRN complex in the IR-induced foci (IRIF). |
| Construction | Recombinant Antibody |
| Purification | Affinity-chromatography |
| Appearance | Liquid |
| Formulation | Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| Research Background | Transcriptional activator which regulates the transcription of various genes, including those involved in anti-apoptosis, cell growth, and DNA damage response. Dependent on its binding partner, binds to CRE (cAMP response element) consensus sequences (5'-TGACGTCA-3') or to AP-1 (activator protein 1) consensus sequences (5'-TGACTCA-3'). In the nucleus, contributes to global transcription and the DNA damage response, in addition to specific transcriptional activities that are related to cell development, proliferation and death. In the cytoplasm, interacts with and perturbs HK1- and VDAC1-containing complexes at the mitochondrial outer membrane, thereby impairing mitochondrial membrane potential, inducing mitochondrial leakage and promoting cell death. The phosphorylated form (mediated by ATM) plays a role in the DNA damage response and is involved in the ionizing radiation (IR)-induced S phase checkpoint control and in the recruitment of the MRN complex into the IR-induced foci (IRIF). Exhibits histone acetyltransferase (HAT) activity which specifically acetylates histones H2B and H4 in vitro. In concert with CUL3 and RBX1, promotes the degradation of KAT5 thereby attenuating its ability to acetylate and activate ATM. Can elicit oncogenic or tumor suppressor activities depending on the tissue or cell type. |
| Conjucates | Unconjugated |
| Immunogen | A synthetic peptide: Human Phospho-ATF2 (T71) |
| Antigen Species | Human |
| Gene ID | 1386 |
| Uniprot ID | |
| Biology Area | Epigenetics and Nuclear Signaling |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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