Antibody Type: Recombinant Rabbit Monoclonal

Application: ELISA, WB, IF, FCM

Reactivity: Human

Rabbit IgG

8Z224

Human

1. Western Blot
-Positive WB detected in: HT29 whole cell lysate, U87 whole cell lysate,Hela whole cell lysate,A549 whole cell lysate,HEPG2 whole cell lysate
-All lanes: Syntenin antibody at 1:1000
-Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution
-Predicted band size: 32 kDa
-Observed band size: 32 kDa
2. Immunofluorescence staining of Hela with TMAH-01077 at 1:30, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 521-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
3. Overlay Peak curve showing HepG2 cells stained with TMAH-01077 (red line) at 1:50. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1µg/1*10^6 cells) for 45min at 4°C. The secondary antibody used was FITC-conjugated Goat Anti-rabbit IgG(H+L) at 1:200 dilution for 35min at 4°C.Control antibody (green line) was rabbit IgG (1µg/1*10^6 cells) used under the same conditions. Acquisition of >10,000 events was performed.

ELISA, WB, IF, FCM

WB:1:500-1:2000; IF:1:50-1:200; FCM:1:50-1:200.

Monoclonal

Unconjugated

A synthetic peptide: Human SDCBP

Human

6386

Neuroscience