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Anti-Osteopontin Antibody (2F952)

😃Good
Catalog No. TMAH-01130
Alias secreted phosphoprotein 1, OPN, ETA-1, BSPI, BNSP

Anti-Osteopontin Antibody (2F952) is an antibody targeting Osteopontin. Anti-Osteopontin Antibody (2F952) can be used in ELISA, FCM.

Anti-Osteopontin Antibody (2F952)

Anti-Osteopontin Antibody (2F952)

😃Good
Catalog No. TMAH-01130Alias secreted phosphoprotein 1, OPN, ETA-1, BSPI, BNSP
Anti-Osteopontin Antibody (2F952) is an antibody targeting Osteopontin. Anti-Osteopontin Antibody (2F952) can be used in ELISA, FCM.
Pack SizePriceAvailabilityQuantity
50 μL$207 7-10 days
100 μL$347 7-10 days
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Product Introduction

Bioactivity
Description
Antibody Type: Recombinant Mouse Monoclonal

Application: ELISA, FCM

Reactivity: Human
Aliassecreted phosphoprotein 1, OPN, ETA-1, BSPI, BNSP
Ig Type
Mouse IgG2a
Clone
2F952
Reactivity
Human
Verified Activity
Overlay Peak curve showing A549 cells stained with TMAH-01130 (red line) at 1:100. Then 10% normal goat serum was Incubated to block non-specific protein-protein interactions followed by the antibody (1µg/1*10^6 cells) for 45 min at 4°C. The secondary antibody used was FITC-conjugated Goat Anti-Mouse IgG(H+L) at 1/200 dilution for 35 min at 4°C. Isotype control antibody (green line) was mouse IgG1 (1µg/1*10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.
Application
ELISA, FCM
Recommended Dose
FCM:1:20-1:500.
Antibody Type
Monoclonal
Subcellular LocalizationSecreted.
ConstructionRecombinant Antibody
PurificationAffinity-chromatography
AppearanceLiquid
FormulationPreservative: 0.03% Proclin 300. Constituents: 50% Glycerol, 0.01M PBS, PH 7.4.
Research BackgroundMajor non-collagenous bone protein that binds tightly to hydroxyapatite. Appears to form an integral part of the mineralized matrix. Probably important to cell-matrix interaction. Acts as a cytokine involved in enhancing production of interferon-gamma and interleukin-12 and reducing production of interleukin-10 and is essential in the pathway that leads to type I immunity.
Related Conjugates and Formulations
Conjucates
Unconjugated
Antigen Details
Immunogen
Recombinant Protein: Human SPP1 Protein
Antigen Species
Human
Gene ID
6696
Uniprot ID
Biology Area
Cancer, Cardiovascular, Signal transduction, Stem cells
Chemical Properties
Stability & Storage
Stability & StorageStore at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

Sci Citations

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Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc

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