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Anti-Osteopontin Antibody (2F952) is an antibody targeting Osteopontin. Anti-Osteopontin Antibody (2F952) can be used in ELISA, FCM.
Pack Size | Price | Availability | Quantity |
---|---|---|---|
50 μL | $207 | 7-10 days | |
100 μL | $347 | 7-10 days |
Description | Antibody Type: Recombinant Mouse Monoclonal Application: ELISA, FCM Reactivity: Human |
Alias | secreted phosphoprotein 1, OPN, ETA-1, BSPI, BNSP |
Ig Type | Mouse IgG2a |
Clone | 2F952 |
Reactivity | Human |
Verified Activity | Overlay Peak curve showing A549 cells stained with TMAH-01130 (red line) at 1:100. Then 10% normal goat serum was Incubated to block non-specific protein-protein interactions followed by the antibody (1µg/1*10^6 cells) for 45 min at 4°C. The secondary antibody used was FITC-conjugated Goat Anti-Mouse IgG(H+L) at 1/200 dilution for 35 min at 4°C. Isotype control antibody (green line) was mouse IgG1 (1µg/1*10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed. |
Application | ELISA, FCM |
Recommended Dose | FCM:1:20-1:500. |
Antibody Type | Monoclonal |
Subcellular Localization | Secreted. |
Construction | Recombinant Antibody |
Purification | Affinity-chromatography |
Appearance | Liquid |
Formulation | Preservative: 0.03% Proclin 300. Constituents: 50% Glycerol, 0.01M PBS, PH 7.4. |
Research Background | Major non-collagenous bone protein that binds tightly to hydroxyapatite. Appears to form an integral part of the mineralized matrix. Probably important to cell-matrix interaction. Acts as a cytokine involved in enhancing production of interferon-gamma and interleukin-12 and reducing production of interleukin-10 and is essential in the pathway that leads to type I immunity. |
Conjucates | Unconjugated |
Immunogen | Recombinant Protein: Human SPP1 Protein |
Antigen Species | Human |
Gene ID | 6696 |
Uniprot ID | |
Biology Area | Cancer, Cardiovascular, Signal transduction, Stem cells |
Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
Transport | Shipping with blue ice. |
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