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| Pack Size | Price | USA Stock | Global Stock | Quantity |
|---|---|---|---|---|
| 5 μg | $975 | 7-10 days | 7-10 days | |
| 10 μg | $1,650 | 7-10 days | 7-10 days | |
| 20 μg | $2,800 | 7-10 days | 7-10 days | |
| 50 μg | $5,720 | 7-10 days | 7-10 days | |
| 100 μg | $9,830 | 7-10 days | 7-10 days |
| Description | A2AR Protein-Nanodisc, Human, Recombinant (His & Flag) is expressed in HEK293 Cells. The accession number is P29274. |
| Species | Human |
| Expression System | HEK293 Cells |
| Tag | N-Flag, C-His |
| Accession Number | P29274 |
| Construction | A DNA sequence encoding the human AA2AR (P29274) (Pro2-Leu208 & Glu219-Ala316) (TM domains 1-7) was expressed and linked by a Fusion Protein, with a Flag tag at the N-terminus and a polyhistidine tag at the C-terminus. Nanodisc is a versatile tool for studying membrane proteins. Using styrene-maleic acid (SMA) copolymer, membrane proteins can be extracted directly from prokaryotic and eukaryotic expression systems in the absence of detergents to preserve the protein structure and function better. Compared to membrane scaffold proteins (MSPs) nanodiscs, SMA nanodiscs also have the advantage of preserving proteins' nature by maintaining native lipids surrounded without introducing any heterologous proteins, which allows studies of protein structure and functions in a native-like environment. |
| Protein Purity | ≥ 95% as determined by SDS-PAGE. |
| Endotoxin | < 1.0 EU/μg of the protein as determined by the LAL method. |
| Formulation | Supplied as sterile 10mM Hepes, 50mM NaCl, pH 7.5. Please contact us for any concerns or special requirements. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA. |
| Stability & Storage | Samples are stable for up to three months from date of receipt at -70℃. Store it under sterile conditions at -70℃ or lower. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. |
| Shipping | Proteins are shipped with blue ice. |
| Research Background |
| Molecular Weight | 11.83 kDa (predicted); 41 kDa (reducing contition) |
| Storage | Lyophilized powder: -20~-80°C for 1 year | Solution: -80°C for 6 months |
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