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SEMA4D Protein, Human, Recombinant (His & Avi)

(Synonyms: Semaphorin-4D, SEMAJ, SEMA4D, M-sema-G, MGC169141, MGC169138, FLJ46484, FLJ39737, FLJ34282, FLJ33485, coll-4, CD100, C9orf164) Copy Product Info

Synonyms: Semaphorin-4D, SEMAJ, SEMA4D, M-sema-G, MGC169141, MGC169138, FLJ46484, FLJ39737, FLJ34282, FLJ33485, coll-4, CD100, C9orf164

Catalog No. TMPK-00403 Copy Product Info
Semaphorin 4D (Sema4D) is a multifunctional protein widely expressed in an organism that plays an important role in the control of many physiological and pathological processes, including immunoregulation, neurogenesis, angiogenesis, and tumor progression. SEMA4D Protein, Human, Recombinant (His & Avi) is expressed in HEK293 mammalian cells with C-His-Avi tag. The predicted molecular weight is 82.1 kDa and the accession number is Q92854-1.
Pack SizePriceUSA StockGlobal StockQuantity
5 μg$567-10 days7-10 days
10 μg$887-10 days7-10 days
20 μg$1437-10 days7-10 days
50 μg$2787-10 days7-10 days
100 μg$4657-10 days7-10 days
200 μg$8397-10 days7-10 days
500 μg$1,8507-10 days7-10 days
For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)
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For research use only—not for human use. No sales to individuals. Use as intended only.
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Product Introduction

Bioactivity
Bioactivity
Immobilized Human Semaphorin 4D, His Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Anti-Semaphorin 4D Antibody , hFc Tag with the EC50 of 9.3ng/ml determined by ELISA.
Description
Semaphorin 4D (Sema4D) is a multifunctional protein widely expressed in an organism that plays an important role in the control of many physiological and pathological processes, including immunoregulation, neurogenesis, angiogenesis, and tumor progression. SEMA4D Protein, Human, Recombinant (His & Avi) is expressed in HEK293 mammalian cells with C-His-Avi tag. The predicted molecular weight is 82.1 kDa and the accession number is Q92854-1.
Species
Human
Expression System
HEK293 Cells
TagC-His-Avi
Accession NumberQ92854-1
ConstructionMet22-Arg734
Protein Purity
> 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC
Endotoxin< 1.0 EU/μg of the protein as determined by the LAL method.
FormulationLyophilized from a solution filtered through a 0.22 μm filter, containing PBS (pH 7.4). Typically, 8% trehalose is incorporated as a protective agent before lyophilization.
ReconstitutionReconstitute the lyophilized protein in distilled water. The product concentration should not be less than 100 μg/ml. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing.
Stability & StorageIt is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
ShippingIn general, lyophilized powders are shipped with blue ice, while solutions are shipped with dry ice.
SynonymsSemaphorin-4D, SEMAJ, SEMA4D, M-sema-G, MGC169141, MGC169138, FLJ46484, FLJ39737, FLJ34282, FLJ33485, coll-4, CD100, C9orf164
Research Background
Chemical Properties
Molecular Weight82.1 kDa (predicted). Due to glycosylation, the protein migrates to 115-140 kDa based on Tris-Bis PAGE result.
Storage & Solubility Information
StorageLyophilized powder: -20~-80°C for 1 year | Solution: -80°C for 6 months

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Dose Conversion

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Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc

Keywords

Related Tags: SEMA4D Protein, Human, Recombinant (His & Avi) chemical structure | SEMA4D Protein, Human, Recombinant (His & Avi) molecular weight