Shopping Cart
- Remove All
Your shopping cart is currently empty
Anti-CD34 Antibody (1D438)
😃Good
Catalog No. TMAY-03369
Alias CD34 molecule, AU040960
Anti-CD34 Antibody (1D438) is a Rabbit antibody targeting CD34. Anti-CD34 Antibody (1D438) can be used in ELISA,ELISA(Det),IHC-P,FCM,ICC/IF,IP.
Anti-CD34 Antibody (1D438)
Anti-CD34 Antibody (1D438)
😃Good
Catalog No. TMAY-03369Alias CD34 molecule, AU040960
Anti-CD34 Antibody (1D438) is a Rabbit antibody targeting CD34. Anti-CD34 Antibody (1D438) can be used in ELISA,ELISA(Det),IHC-P,FCM,ICC/IF,IP.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 20 μL | $93 | 7-10 days | |
| 100 μL | $268 | 7-10 days |
Bulk & Custom
Add to Cart
Questions
View MoreContact us for more batch information
Resource Download
Product Introduction
Bioactivity
Related Conjugates and Formulations
Antigen Details
Chemical Properties
| Description | Anti-CD34 Antibody (1D438) is a Rabbit antibody targeting CD34. Anti-CD34 Antibody (1D438) can be used in ELISA,ELISA(Det),IHC-P,FCM,ICC/IF,IP. |
| Alias | CD34 molecule, AU040960 |
| Ig Type | Monoclonal Rabbit IgG |
| Clone | 1D438 |
| Reactivity | Mouse |
| Verified Activity | 1. Immunochemical Staining of mouse CD34 in mouse stomach with Rabbit Monoclonal Antibody at 1:5000 dilution, formalin-fixed paraffin embedded sections. Positive staining was localized to vascular endothelial cells. 2. Immunochemical Staining of mouse CD34 in mouse kidney with Rabbit Monoclonal Antibody at 1:5000 dilution, formalin-fixed paraffin embedded sections. Positive staining was localized to vascular endothelial cells. 3. Flow cytometric analysis of CD34 expression on M1 cells. Cells were stained with purified anti-Mouse CD34, then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells. 4. Mouse CD34 was immunoprecipitated using: -Lane A:0.5 mg Hela Whole Cell Lysate. -Lane B:0.5 mg 293T Whole Cell Lysate -0.5 µL anti-Mouse CD34 rabbit monoclonal antibody and 15 μl of 50 % Protein G agarose. -Primary antibody: -Anti-Mouse CD34 rabbit monoclonal antibody, at 1:350 dilution. -Secondary antibody: -Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution. -Developed using the odyssey technique. -Performed under reducing conditions. -Predicted band size: 41 kDa. -Observed band size: 130 kDa. 5. Immunofluorescence staining of CD34 in M1 cells. Cells were fixed with 4% PFA, blocked with 10% serum, and incubated with rabbit anti-mouse CD34 monoclonal antibody (1:300) at 4°C overnight. Then cells were stained with the Alexa Fluor® 488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue). |
| Application | ELISA,ELISA(Det),IHC-P,FCM,ICC/IF,IP |
| Recommended Dose | ELISA: 1:25000-1:50000; IHC-P: 1:2000-1:10000; ICC-IF: 1:100-1:500; FCM: 1:100-1:500; IP: 0.2-1 μL/mg of lysate; ELISA(Det): 1:5000-1:50000 |
| Antibody Type | Monoclonal |
| Host Species | Rabbit |
| Construction | This antibody was obtained from a rabbit immunized with purified, recombinant Mouse CD34 (rM CD34; TMPY-01652; NP_001104529.1; Met 1-Thr 287). |
| Purification | Protein A |
| Appearance | Liquid |
| Formulation | 0.2 μm filtered solution in PBS |
| Research Background | Cluster of Differentiation 34 (CD34) is a member of a family of single-pass transmembrane sialomucin proteins, and may function as a cell-cell adhesion factor. CD34 protein is selectively expressed on hematopoietic progenitor cells and the small vessel endothelium of a variety of tissues. It has been widely used as a stem and progenitor cell marker, and clinical CD34+ stem cell transplantation (CD34+SCT) has been performed for tumor purging. CD34 monoclonal antibodies are widely used to identify and isolate hemopoietic progenitors and to classify acute and chronic leukemias. |
| Conjucates | Unconjugated |
| Others Formats | APC/PerCP/FITC/PE |
| Antibody Types Available | 5 |
| Immunogen | Recombinant Mouse CD34 Protein (TMPY-01652) |
| Antigen Species | Mouse |
| Biology Area | Cardiac Stem Cell Markers, Hemangioblast Markers |
Stability & Storage
| Stability & Storage | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free. |
| Transport | Shipping with blue ice. |
Sci Citations
Calculator
For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . 
Tech Support
Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
Preview
Related Tags: buy Anti-CD34 Antibody (1D438) | purchase Anti-CD34 Antibody (1D438) | Anti-CD34 Antibody (1D438) cost | order Anti-CD34 Antibody (1D438)
- TOP
Feedback
Hello! How can I help you today? 
Copyright © 2015-2025 TargetMol Chemicals Inc. All Rights Reserved.