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Anti-RhoA Antibody (5G87)
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Catalog No. TMAH-01043
Alias RHOH12, RHO12, ras homolog family member A, ARHA, ARH12
Anti-RhoA Antibody (5G87) is an antibody targeting RhoA. Anti-RhoA Antibody (5G87) can be used in ELISA, WB.
Anti-RhoA Antibody (5G87)
Anti-RhoA Antibody (5G87)
😃Good
Catalog No. TMAH-01043Alias RHOH12, RHO12, ras homolog family member A, ARHA, ARH12
Anti-RhoA Antibody (5G87) is an antibody targeting RhoA. Anti-RhoA Antibody (5G87) can be used in ELISA, WB.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 50 μL | $207 | 7-10 days | |
| 100 μL | $349 | 7-10 days |
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Product Introduction
Bioactivity
Related Conjugates and Formulations
Antigen Details
Chemical Properties
| Description | Antibody Type: Recombinant Rabbit Monoclonal Application: ELISA, WB Reactivity: Human |
| Alias | RHOH12, RHO12, ras homolog family member A, ARHA, ARH12 |
| Ig Type | Rabbit IgG |
| Clone | 5G87 |
| Reactivity | Human |
| Verified Activity | Western Blot -Positive WB detected in: SH-SY5Y whole cell lysate -All lanes: Rho antibody at 1:1500 -Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution -Predicted band size: 22 kDa -Observed band size: 22 kDa |
| Application | ELISA, WB |
| Recommended Dose | WB:1:500-1:5000. |
| Antibody Type | Monoclonal |
| Subcellular Localization | Cell membrane; Lipid-anchor; Cytoplasmic side. Cytoplasm, cytoskeleton. Cleavage furrow. Cytoplasm, cell cortex. Midbody. Cell projection, lamellipodium. Cell projection, dendrite. |
| Construction | Recombinant Antibody |
| Purification | Affinity-chromatography |
| Appearance | Liquid |
| Formulation | Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| Research Background | Small GTPase which cycles between an active GTP-bound and an inactive GDP-bound state. Mainly associated with cytoskeleton organization, in active state binds to a variety of effector proteins to regulate cellular responses such as cytoskeletal dynamics, cell migration and cell cycle. Regulates a signal transduction pathway linking plasma membrane receptors to the assembly of focal adhesions and actin stress fibers. Involved in a microtubule-dependent signal that is required for the myosin contractile ring formation during cell cycle cytokinesis. Plays an essential role in cleavage furrow formation. Required for the apical junction formation of keratinocyte cell-cell adhesion. Essential for the SPATA13-mediated regulation of cell migration and adhesion assembly and disassembly. The MEMO1-RHOA-DIAPH1 signaling pathway plays an important role in ERBB2-dependent stabilization of microtubules at the cell cortex. It controls the localization of APC and CLASP2 to the cell membrane, via the regulation of GSK3B activity. In turn, membrane-bound APC allows the localization of the MACF1 to the cell membrane, which is required for microtubule capture and stabilization. Regulates KCNA2 potassium channel activity by reducing its location at the cell surface in response to CHRM1 activation; promotes KCNA2 endocytosis. Acts as an allosteric activator of guanine nucleotide exchange factor ECT2 by binding in its activated GTP-bound form to the PH domain of ECT2 which stimulates the release of PH inhibition and promotes the binding of substrate RHOA to the ECT2 catalytic center. May be an activator of PLCE1. In neurons, involved in the inhibiton of the initial spine growth. Upon activation by CaMKII, modulates dendritic spine structural plasticity by relaying CaMKII transient activation to synapse-specific, long-term signaling. Acts as a regulator of platelet alpha-granule release during activation and aggregation of platelets. (Microbial infection) Serves as a target for the yopT cysteine peptidase from Yersinia pestis, vector of the plague. |
| Conjucates | Unconjugated |
| Immunogen | A synthetic peptide: Human Rho |
| Antigen Species | Human |
| Gene ID | 387 |
| Uniprot ID | |
| Biology Area | Cancer, Signal transduction |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
Sci Citations
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For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . 
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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