Assay #1: ED 50 < 0.1 μg/ml, measured by the neutralization assay using 929 cells in presence of 0.25 ng/ml of human TNF-beta, corresponding to a specific activity of > 1.0 × 10 4 units/mg. Assay #2: Immobilized HVEM, hFc, Human at 2.0 μg/ml (100 µl/well) can bind biotinylated human BTLA. Assay #3: Immobilized HVEM, hFc, Human at 2.0 μg/ml (100 µl/well) can bind biotinylated CD160, hFc, Human (Cat. No.: Z03449).

HVEM Protein, Human, Recombinant (Insect, hFc) is expressed in Baculovirus Insect Cells. The accession number is Q92956.

Human

Baculovirus Insect Cells

TR2,TNFRSF14

Leu39-Lys184

> 95% as determined by SDS-PAGE; > 95% as determined by HPLC

Reconstitute the lyophilized protein in sterile deionized water. The product concentration should not be less than 100 μg/ml. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing.

Upon receiving, this product remains stable for up to 6 months at lower than -70°C. Upon reconstitution, the product should be stable for up to 1 week at 4°C or up to 3 months at -20°C. For long term storage it is recommended that a carrier protein (example 0.1% BSA) be added. Avoid repeated freeze-thaw cycles.

Herpes Virus Entry Mediator (HVEM) is a transmembrane protein that is the receptor for TNFSF14 (also known as LIGHT) and is therefore referred to asTNFRSF14. HVEM is expressed broadly on immune cells such as T cells, natural killer (NK) cells and monocytes. The interaction of 3 molecules of LIGHT with three molecules of HVEM forms a hexameric complex that leads to the recruitment and retention of effector cells and activates NK cells to produce large amounts of IFN-γ and GM-CSF. In addition to the canonical binding partner LIGHT, HVEM can also bind to the inhibitory signaling protein, B- and T- lymphocyte attenuator (BTLA), which suppresses immune responses. Therefore, the HVEM network plays an important role in regulating immunity and the behavior of lymphocytes.