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Anti-ANXA1 Antibody (3E707)
😃Good
Catalog No. TMAH-00063
Alias p35, LPC1, Lipocortin I, Chromobindin-9, Calpactin-2, Calpactin II, ANXA1, ANX1, Annexin-1, Annexin I, Annexin A1
Anti-ANXA1 Antibody (3E707) is an antibody targeting ANXA1. Anti-ANXA1 Antibody (3E707) can be used in ELISA, IHC, FC.
Anti-ANXA1 Antibody (3E707)
Anti-ANXA1 Antibody (3E707)
😃Good
Catalog No. TMAH-00063Alias p35, LPC1, Lipocortin I, Chromobindin-9, Calpactin-2, Calpactin II, ANXA1, ANX1, Annexin-1, Annexin I, Annexin A1
Anti-ANXA1 Antibody (3E707) is an antibody targeting ANXA1. Anti-ANXA1 Antibody (3E707) can be used in ELISA, IHC, FC.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 50 μL | $207 | 7-10 days | |
| 100 μL | $348 | 7-10 days |
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Product Introduction
Bioactivity
Related Conjugates and Formulations
Antigen Details
Chemical Properties
| Description | Antibody Type: Recombinant Monoclonal Application: ELISA, IHC, FC Reactivity: Human |
| Alias | p35, LPC1, Lipocortin I, Chromobindin-9, Calpactin-2, Calpactin II, ANXA1, ANX1, Annexin-1, Annexin I, Annexin A1 |
| Ig Type | Mouse IgG2a |
| Clone | 3E707 |
| Reactivity | Human |
| Verified Activity | 1. IHC image of TMAH-00063 diluted at 1:30 and staining in paraffin-embedded human liver cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-Mouse IgG labeled by HRP and visualized using 0.05% DAB. 2. Overlay Peak curve showing MCF7 cells stained with TMAH-00063 (red line) at 1:400. The cells were fixed in 4% formaldehyde (15min) and permeated by 0.2% TritonX-100 for 10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*10^6 cells) for 45min at 4°C. The secondary antibody used was FITC-conjugated Goat Anti-Mouse IgG(H+L) at 1/200 dilution for 35 min at 4°C. Isotype control antibody (green line) was mouse IgG1 (1µg/1*10^6 cells) used under the same conditions. Acquisition of >10,016 events was performed. |
| Application | ELISA, IHC, FC |
| Recommended Dose | IHC:1:20-1:200; FC:1:20-1:200. |
| Antibody Type | Monoclonal |
| Subcellular Localization | Nucleus. Cytoplasm. Cell projection, cilium. Cell membrane. Membrane; Peripheral membrane protein. Endosome membrane; Peripheral membrane protein. Basolateral cell membrane. Apical cell membrane. Lateral cell membrane. Secreted. Secreted, extracellular space. Cell membrane; Peripheral membrane protein; Extracellular side. Secreted, extracellular exosome. Cytoplasmic vesicle, secretory vesicle lumen. Cell projection, phagocytic cup. Early endosome. Cytoplasmic vesicle membrane; Peripheral membrane protein. |
| Construction | Recombinant Antibody |
| Purification | Affinity-chromatography |
| Appearance | Liquid |
| Formulation | Preservative: 0.03% Proclin 300. Constituents: 50% Glycerol, 0.01M PBS, PH 7.4. |
| Research Background | Plays important roles in the innate immune response as effector of glucocorticoid-mediated responses and regulator of the inflammatory process. Has anti-inflammatory activity. Plays a role in glucocorticoid-mediated down-regulation of the early phase of the inflammatory response. Promotes resolution of inflammation and wound healing. Functions at least in part by activating the formyl peptide receptors and downstream signaling cascades. Promotes chemotaxis of granulocytes and monocytes via activation of the formyl peptide receptors. Contributes to the adaptive immune response by enhancing signaling cascades that are triggered by T-cell activation, regulates differentiation and proliferation of activated T-cells. Promotes the differentiation of T-cells into Th1 cells and negatively regulates differentiation into Th2 cells. Has no effect on unstimulated T cells. Promotes rearrangement of the actin cytoskeleton, cell polarization and cell migration. Negatively regulates hormone exocytosis via activation of the formyl peptide receptors and reorganization of the actin cytoskeleton. Has high affinity for Ca(2+) and can bind up to eight Ca(2+) ions. Displays Ca(2+)-dependent binding to phospholipid membranes. Plays a role in the formation of phagocytic cups and phagosomes. Plays a role in phagocytosis by mediating the Ca(2+)-dependent interaction between phagosomes and the actin cytoskeleton. |
| Conjucates | Unconjugated |
| Immunogen | Recombinant Protein: Human ANXA1 Protein |
| Antigen Species | Human |
| Gene ID | 301 |
| Uniprot ID | |
| Biology Area | Signal transduction |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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