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Anti-14-3-3 beta Antibody (9I180)

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Catalog No. TMAY-01251
Alias tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, β, tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, beta, 14-3-3 β

Anti-14-3-3 beta Antibody (9I180) is a Mouse antibody targeting 14-3-3 beta. Anti-14-3-3 beta Antibody (9I180) can be used in ELISA,FCM,ICC/IF.

Anti-14-3-3 beta Antibody (9I180)

Anti-14-3-3 beta Antibody (9I180)

😃Good
Catalog No. TMAY-01251Alias tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, β, tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, beta, 14-3-3 β
Anti-14-3-3 beta Antibody (9I180) is a Mouse antibody targeting 14-3-3 beta. Anti-14-3-3 beta Antibody (9I180) can be used in ELISA,FCM,ICC/IF.
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100 μL$198 7-10 days
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Product Introduction

Bioactivity
Description
Anti-14-3-3 beta Antibody (9I180) is a Mouse antibody targeting 14-3-3 beta. Anti-14-3-3 beta Antibody (9I180) can be used in ELISA,FCM,ICC/IF.
Aliastyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, β, tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, beta, 14-3-3 β
Ig Type
Monoclonal Mouse IgG2b
Clone
9I180
Reactivity
Human
Verified Activity
1. Immunofluorescence staining of Human YWHAB in MCF7 cells. Cells were fixed with 4% PFA, blocked with 10% serum, and incubated with Mouse anti-Human YWHAB monoclonal antibody (1:60) at 4°C overnight. Then cells were stained with the Alexa Fluor® 488-conjugated Goat Anti-mouse IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to cytoplasm.

2. Flow cytometric analysis of Human YWHAB expression on Molt-4 cells. The cells were treated according to manufacturer’s manual (BD Pharmingen™ Cat. No. 554714), stained with purified anti-Human YWHAB, then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.
Application
ELISA,FCM,ICC/IF
Recommended Dose
ELISA: 1:1000-1:2000; ICC-IF: 1:20-1:100; FCM: 1:25-1:100
Antibody Type
Monoclonal
Host SpeciesMouse
ConstructionThis antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human 14-3-3 beta / YWHAB (rh 14-3-3 beta / YWHAB; TMPY-01266; NP_003395.1; Met1-Asn246). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
PurificationProtein A
AppearanceLiquid
Formulation0.2 μm filtered solution in PBS
Research Background14-3-3 beta / YWHAB is a member of the 14-3-3 proteins family. 14-3-3 proteins are a group of highly conserved proteins that are involved in many vital cellular processes such as metabolism, protein trafficking, signal transduction, apoptosis and cell cycle regulation. 14-3-3 proteins are mainly localized in the synapses and neuronal cytoplasm, and seven isoforms have been identified in mammals. This family of proteins was initially identified as adaptor proteins which bind to phosphoserine-containing motifs. Binding motifs and potential functions of 14-3-3 proteins are now recognized to have a wide range of functional relevance. 14-3-3 beta / YWHAB is found in both plants and mammals, and this protein is 100% identical to the mouse ortholog. 14-3-3 beta / YWHAB interacts with CDC25 phosphatases, RAF1 and IRS1 proteins, suggesting its role in diverse biochemical activities related to signal transduction, such as cell division and regulation of insulin sensitivity. 14-3-3 beta / YWHAB has also been implicated in the pathogenesis of small cell lung cancer. 14-3-3 beta / YWHAB binding negatively regulates RSK1 activity to maintain signal specificity and that association/dissociation of the 14-3-3beta-RSK1 complex is likely to be important for mitogen-mediated RSK1 activation.
Related Conjugates and Formulations
Conjucates
Unconjugated
Others Formats
APC/PerCP/FITC/PE
Antibody Types Available
5
Antigen Details
Immunogen
Recombinant Human 14-3-3 beta / YWHAB protein (TMPY-01266)
Antigen Species
Human
Biology Area
phospho-Serine/phospho-Threonine Binding Proteins, Adaptor Proteins, Apoptosis Adaptor Proteins
Chemical Properties
Stability & Storage
Stability & StorageStore at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free.
TransportShipping with blue ice.

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Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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