VEGFC Protein, Human, Recombinant (His)

Name: VEGFC Protein, Human, Recombinant (His)
Brand: TargetMol
SKU: TMPY-01007
Price: 57 USD
Availability: InStock
Rating: 5 (10 reviews)

(Synonyms: VRP, VEGF-C, vascular endothelial growth factor C, LMPH1D, Flt4-L) Copy Product Info

Synonyms: VRP, VEGF-C, vascular endothelial growth factor C, LMPH1D, Flt4-L

Catalog No. TMPY-01007 Copy Product Info

Purity: 99.4%

VEGFC Protein, Human, Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 15.5 kDa and the accession number is P49767.

Pack Size	Price	USA Stock	Global Stock
5 µg	$57	-	In Stock
10 µg	$83	7-10 days	7-10 days
20 µg	$122	7-10 days	7-10 days
50 µg	$201	7-10 days	7-10 days
100 µg	$296	-	In Stock

For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)

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For research use only—not for human use. No sales to individuals. Use as intended only.

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Purity:99.4%

Resource Download

COA User Guide of Recombinant Proteins

Product Introduction

Bioactivity

Bioactivity	1. Measured by its binding ability in a functional ELISA. Immobilized VEGF C-his at 2 μg/mL (100 μL/well) can bind VEGFR3 hFc, the EC50 of VEGFR3 hFc is 2-15 ng/mL. 2. Scatchard analysis showed the affinity constant (Kd) of recombinant human VEGF-C bound to recombinant human VEGFR3 was 1.4 nM. 3. Measured in a cell proliferation assay using human umbilical vein endothelial cells (HUVEC). The ED50 for this effect is typically 0.1-0.5 μg/mL. 4. Captured VEGFR3/FLT4 Protein, Human on anti-Human IgG Fc via CM5 Chip can bind VEGF-C with an affinity constant of 31.82 pM as determined in a SPR assay (Routinely tested).
Description	VEGFC Protein, Human, Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 15.5 kDa and the accession number is P49767.
Species	Human
Expression System	HEK293 Cells
Tag	C-His
Accession Number	P49767
Construction	A DNA sequence encoding the mature form of human VEGFC (NP_005420.1) (Thr103-Arg227) was expressed with a C-terminal polyhistidine tag. Predicted N terminal: Thr 103
Protein Purity	> 95 % as determined by SDS-PAGE
Endotoxin	< 1.0 EU/μg of the protein as determined by the LAL method.
Formulation	Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH 7.4. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
Reconstitution	It is recommended that sterile water (50μl) be added to the vial to prepare a stock solution of 0.1mg/mL. Reconstitution conditions may vary depending on the lot.
Synonyms	VRP, VEGF-C, vascular endothelial growth factor C, LMPH1D, Flt4-L
Research Background	Vascular endothelial growth factor C (VEGF-C) is a member of the VEGF family. Upon biosynthesis, VEGF-C protein is secreted as a non-covalent momodimer in an anti-parellel fashion. VEGF-C protein is a dimeric glycoprotein, as a ligand for two receptors, VEGFR-3 (Flt4), and VEGFR-2. VEGF-C may function in angiogenesis of the venous and lymphatic vascular systems during embryogenesis. VEGF-C protein is over-expressed in various human cancers including breast cancer and prostate cancer. VEGF-C/VEGFR-3 axis, through different signaling pathways, plays a critical role in cancer progression by regulating different cellular functions, such as invasion, proliferation, and resistance to chemotherapy. Thus, targeting the VEGF-C/VEGFR-3 axis may be therapeutically significant for certain types of tumors.

Chemical Properties

Molecular Weight

15.5 kDa (predicted); 22.5 kDa (reducing condition, due to glycosylation)

Storage & Solubility Information

Shipping	In general, lyophilized powders are shipped with blue ice, while solutions are shipped with dry ice.
Storage	It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.