CD3 epsilon/CD3e Protein, Human, Recombinant (His) is expressed in CHO mammalian cells with His tag. The predicted molecular weight is 13.2 kDa and the accession number is P07766.
Pack Size | Availability | Price/USD | Quantity |
---|---|---|---|
50 μg | 5 days | $ 498.00 | |
500 μg | 5 days | $ 2,510.00 |
Description | CD3 epsilon/CD3e Protein, Human, Recombinant (His) is expressed in CHO mammalian cells with His tag. The predicted molecular weight is 13.2 kDa and the accession number is P07766. |
Species | Human |
Expression System | CHO Cells |
Tag | C-His |
Accession Number | P07766 |
Synonyms | CD3e molecule, epsilon (CD3-TCR complex), CD3 ε/CD3e, TCRE, CD3 epsilon, IMD18, CD3 ε, CD3e molecule, ε (CD3-TCR complex), T3E |
Construction | The Human CD3E (NP_000724.1) (Met1-Asp126) was expressed with a polyhistidine tag at the C-terminus. |
Protein Purity | > 95 % as determined by SDS-PAGE. |
Molecular Weight | 13.2 kDa (predicted) |
Endotoxin | < 1.0 EU/μg of the protein as determined by the LAL method. |
Formulation | Lyophilized from a solution filtered through a 0.22 μm filter, containing Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH 7.4. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization. |
Reconstitution | A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information. |
Stability & Storage |
It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at-80℃. For reconstituted proteinsolutions, the solution can be stored at -20°c to -80'c for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots. |
Shipping |
In general, Lyophilized powders are shipping with blue ice. |
Research Background | T-cell surface glycoprotein CD3 epsilon chain, also known as CD3E, is a single-pass type I membrane protein. CD3E contains 1 Ig-like (immunoglobulin-like) domain and 1 ITAM domain. CD3E, together with CD3-gamma, CD3-delta and CD3-zeta, and the T-cell receptor alpha/beta and gamma/delta heterodimers, forms the T cell receptor-CD3 complex. The CD3 epsilon subunit of the T cell receptor (TCR) complex contains two defined signaling domains, a proline-rich sequence and an immune tyrosine activation motifs (ITAMs), and this complex undergoes a conformational change upon ligand binding that is thought to be important for the activation of T cells. In the CD3 epsilon mutant mice, all stages of T cell development and activation that are TCR-dependent were impaired, but not eliminated, including activation of mature naïve T cells with the MHCII presented superantigen, staphylococcal enterotoxin B, or with a strong TCR cross-linking antibody specific for either TCR-Cbeta or CD3 epsilon. T cell receptor-CD3 complex plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways. This complex is critical for T-cell development and function, and represents one of the most complex transmembrane receptors. CD3E plays an essential role in T-cell development, and defects in CD3E gene cause severe immunodeficiency. Homozygous mutations in CD3D and CD3E genes lead to a complete block in T-cell development and thus to an early-onset severe combined immunodeficiency phenotype.Cancer ImmunotherapyImmune CheckpointImmunotherapyTargeted Therapy |
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Please read the User Guide of Recombinant Proteins for more specific information.
CD3 epsilon/CD3e Protein, Human, Recombinant (His) CD3e molecule, epsilon CD3e molecule, epsilon (CD3-TCR complex) CD3 ε/CD3e TCRE CD3-TCR complex CD3 epsilon IMD18 CD3 ε CD3e molecule, ε (CD3-TCR complex) T3E IMD-18 IMD 18 recombinant recombinant-proteins proteins protein