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Latent TGF beta 3 Protein, Human, Recombinant (His)

(Synonyms: TGF-β3, TGFβ3, TGF-β, TGFβ, TGF-beta3, TGFB3, Latent TGF β3, ARVD) Copy Product Info

Synonyms: TGF-β3, TGFβ3, TGF-β, TGFβ, TGF-beta3, TGFB3, Latent TGF β3, ARVD

Catalog No. TMPK-00258 Copy Product Info
Transforming growth factor-betas (TGF-βs) are multifunctional cytokines that have been implicated in the regulation of a broad range of biological processes, including cell proliferation, cell survival, and cell differentiation. And transforming growth factor beta3 (TGFbeta3) is a key protein involved in scar-free healing observed in embryos. Latent TGF beta 3 Protein, Human, Recombinant (His) is expressed in HEK293 mammalian cells with N-His tag. The predicted molecular weight is 45.9 kDa and the accession number is P10600-1.

Batch Information

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Purity:> 95% as determined by Bis-Tris PAGE
Appearance:Lyophilized powder
Color:White
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Product Introduction

Bioactivity
Bioactivity
Immobilized Human Latent TGF beta 3, His Tag at 2 μg/mL (100 μL/well) on the plate. Dose response curve for Human TGF-beta RII, mFc Tag with the EC50 of 5.7 ng/mL determined by ELISA (QC Test).
Description
Transforming growth factor-betas (TGF-βs) are multifunctional cytokines that have been implicated in the regulation of a broad range of biological processes, including cell proliferation, cell survival, and cell differentiation. And transforming growth factor beta3 (TGFbeta3) is a key protein involved in scar-free healing observed in embryos. Latent TGF beta 3 Protein, Human, Recombinant (His) is expressed in HEK293 mammalian cells with N-His tag. The predicted molecular weight is 45.9 kDa and the accession number is P10600-1.
Species
Human
Expression System
HEK293 Cells
TagN-His
Accession NumberP10600-1
ConstructionLeu24-Ser412
Protein Purity
> 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC
Endotoxin< 1.0 EU/μg of the protein as determined by the LAL method.
FormulationLyophilized from a solution filtered through a 0.22 μm filter, containing 20 mM PBS (pH 7.4). Typically, 8% trehalose is incorporated as a protective agent before lyophilization.
ReconstitutionReconstitute the lyophilized protein in distilled water. The product concentration should not be less than 100 μg/ml. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing.
SynonymsTGF-β3, TGFβ3, TGF-β, TGFβ, TGF-beta3, TGFB3, Latent TGF β3, ARVD
Research Background
Transforming growth factor-betas (TGF-βs) are multifunctional cytokines that have been implicated in the regulation of a broad range of biological processes, including cell proliferation, cell survival, and cell differentiation. And transforming growth factor beta3 (TGFbeta3) is a key protein involved in scar-free healing observed in embryos.
Chemical Properties
Molecular Weight45.9 kDa (predicted). Due to enzyme lysis and glycosylation, the protein migrates to 46-50 kDa and 55-60 kDa based on Tris-Bis PAGE result.
Storage & Solubility Information
ShippingIn general, lyophilized powders are shipped with blue ice, while solutions are shipped with dry ice.
StorageIt is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.

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Keywords

Related Tags: Latent TGF beta 3 Protein, Human, Recombinant (His) chemical structure | Latent TGF beta 3 Protein, Human, Recombinant (His) molecular weight