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IL-15RA Protein, Human, Recombinant (hFc & Avi), Biotinylated is expressed in HEK293 mammalian cells with hFc and Avi tag. The predicted molecular weight is 46.92 kDa and the accession number is NP_002180.1.
Pack Size | Price | Availability | Quantity |
---|---|---|---|
20 μg | $600 | 7-10 days | |
100 μg | $1,390 | 7-10 days |
Biological Activity | Immobilized Recombinant Human IL-15 / IL15 / Interleukin 15 Protein (His Tag) at 2 μg/ml (100 μl/well) can bind Recombinant Human IL15RA Protein (ECD, Fc & AVI Tag), Biotinylated, The EC50 is 12-36 ng/mL. |
Description | IL-15RA Protein, Human, Recombinant (hFc & Avi), Biotinylated is expressed in HEK293 mammalian cells with hFc and Avi tag. The predicted molecular weight is 46.92 kDa and the accession number is NP_002180.1. |
Species | Human |
Expression System | HEK293 Cells |
Tag | C-hFc-Avi |
Accession Number | NP_002180.1 |
Synonyms | interleukin 15 receptor, α,interleukin 15 receptor, alpha,CD215 |
Construction | A DNA sequence encoding the human IL15RA (NP_002180.1) (Met1-Thr205) was expressed with a c-terminal AVI tagged Fc region of human IgG1 at the C-terminus (Fc-AVI). The expressed protein was biotinylated in vivo by the Biotin-Protein ligase (BirA enzyme) which is co-expressed. Predicted N terminal: Ile 31 |
Protein Purity | > 95 % as determined by SDS-PAGE. > 95 % as determined by SEC-HPLC. |
Molecular Weight | 46.92 kDa (predicted); 73.09 kDa (reducing conditions) |
Endotoxin | < 1.0 EU/μg of the protein as determined by the LAL method. |
Formulation | Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH 7.4. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization. |
Reconstitution | A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information. |
Stability & Storage | It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots. |
Shipping | In general, Lyophilized powders are shipping with blue ice. |
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