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Anti-CD19 Antibody (7Q387)
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Catalog No. TMAH-00177
Alias CVID3, CD19 molecule, B4
Anti-CD19 Antibody (7Q387) is an antibody targeting CD19. Anti-CD19 Antibody (7Q387) can be used in ELISA,IHC,IP,WB.
Anti-CD19 Antibody (7Q387)
Anti-CD19 Antibody (7Q387)
😃Good
Catalog No. TMAH-00177Alias CVID3, CD19 molecule, B4
Anti-CD19 Antibody (7Q387) is an antibody targeting CD19. Anti-CD19 Antibody (7Q387) can be used in ELISA,IHC,IP,WB.
| Pack Size | Price | USA Warehouse | Global Warehouse | Quantity |
|---|---|---|---|---|
| 50 μL | $209 | 7-10 days | 7-10 days | |
| 100 μL | $349 | 7-10 days | 7-10 days |
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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]
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Product Introduction
Bioactivity
Related Conjugates and Formulations
Antigen Details
Chemical Properties
| Description | Anti-CD19 Antibody (7Q387) is an antibody targeting CD19. Anti-CD19 Antibody (7Q387) can be used in ELISA,IHC,IP,WB. |
| Synonyms | CVID3, CD19 molecule, B4 |
| Ig Type | Rabbit IgG |
| Clone | 7Q387 |
| Reactivity | Human |
| Verified Activity | 1. Western Blot -Positive WB detected in: Daudi whole cell lysate, Raji whole cell lysate -All lanes: CD19 antibody at 1:2000 -Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution -Predicted band size: 62 kDa -Observed band size: 95 kDa 2. IHC image of TMAH-00177 diluted at 1:100 and staining in paraffin-embedded human tonsil tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB. 3. Immunoprecipitating CD19 in Raji whole cell lysate -Lane 1: Rabbit control IgG instead of TMAH-00177 in Raji whole cell lysate. For western blotting,a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000) -Lane 2: TMAH-00177(2µg)+ Raji whole cell lysate(500µg) -Lane 3: Raji whole cell lysate (10µg) |
| Application | |
| Recommended Dose | WB:1:500-1:5000; IHC:1:50-1:200; IP:1:200-1:1000. |
| Antibody Type | Monoclonal |
| Subcellular Localization | Cell membrane; Single-pass type I membrane protein. Membrane raft; Single-pass type I membrane protein. |
| Tissue Specificity | Detected on marginal zone and germinal center B cells in lymph nodes. Detected on blood B cells (at protein level). |
| Construction | Recombinant Antibody |
| Purification | Affinity-chromatography |
| Appearance | Liquid |
| Formulation | Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| Research Background | Functions as coreceptor for the B-cell antigen receptor complex (BCR) on B-lymphocytes. Decreases the threshold for activation of downstream signaling pathways and for triggering B-cell responses to antigens. Activates signaling pathways that lead to the activation of phosphatidylinositol 3-kinase and the mobilization of intracellular Ca(2+) stores. Is not required for early steps during B cell differentiation in the blood marrow. Required for normal differentiation of B-1 cells. Required for normal B cell differentiation and proliferation in response to antigen challenges. Required for normal levels of serum immunoglobulins, and for production of high-affinity antibodies in response to antigen challenge. |
| Conjucates | Unconjugated |
| Immunogen | A synthetic peptide: Human CD19 |
| Antigen Species | Human |
| Gene Name | CD19 |
| Gene ID | |
| Uniprot ID | |
| Biology Area | Immunology, Stem cells |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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