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Anti-CD19 Antibody (7Q387)

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Catalog No. TMAH-00177
Alias CVID3, CD19 molecule, B4

Anti-CD19 Antibody (7Q387) is an antibody targeting CD19. Anti-CD19 Antibody (7Q387) can be used in ELISA, WB, IHC, IP.

Anti-CD19 Antibody (7Q387)

Anti-CD19 Antibody (7Q387)

😃Good
Catalog No. TMAH-00177Alias CVID3, CD19 molecule, B4
Anti-CD19 Antibody (7Q387) is an antibody targeting CD19. Anti-CD19 Antibody (7Q387) can be used in ELISA, WB, IHC, IP.
Pack SizePriceAvailabilityQuantity
50 μL$209 7-10 days
100 μL$349 7-10 days
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Product Introduction

Bioactivity
Description
Anti-CD19 Antibody (7Q387) is an antibody targeting CD19. Anti-CD19 Antibody (7Q387) can be used in ELISA, WB, IHC, IP.
AliasCVID3, CD19 molecule, B4
Ig Type
Rabbit IgG
Clone
7Q387
Reactivity
Human
Verified Activity
1. Western Blot
-Positive WB detected in: Daudi whole cell lysate, Raji whole cell lysate
-All lanes: CD19 antibody at 1:2000
-Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution
-Predicted band size: 62 kDa
-Observed band size: 95 kDa
2. IHC image of TMAH-00177 diluted at 1:100 and staining in paraffin-embedded human tonsil tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
3. Immunoprecipitating CD19 in Raji whole cell lysate
-Lane 1: Rabbit control IgG instead of TMAH-00177 in Raji whole cell lysate.
For western blotting,a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)
-Lane 2: TMAH-00177(2µg)+ Raji whole cell lysate(500µg)
-Lane 3: Raji whole cell lysate (10µg)
Application
ELISA, WB, IHC, IP
Recommended Dose
WB:1:500-1:5000; IHC:1:50-1:200; IP:1:200-1:1000.
Antibody Type
Monoclonal
Subcellular LocalizationCell membrane; Single-pass type I membrane protein. Membrane raft; Single-pass type I membrane protein.
ConstructionRecombinant Antibody
PurificationAffinity-chromatography
AppearanceLiquid
FormulationPhosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Research BackgroundFunctions as coreceptor for the B-cell antigen receptor complex (BCR) on B-lymphocytes. Decreases the threshold for activation of downstream signaling pathways and for triggering B-cell responses to antigens. Activates signaling pathways that lead to the activation of phosphatidylinositol 3-kinase and the mobilization of intracellular Ca(2+) stores. Is not required for early steps during B cell differentiation in the blood marrow. Required for normal differentiation of B-1 cells. Required for normal B cell differentiation and proliferation in response to antigen challenges. Required for normal levels of serum immunoglobulins, and for production of high-affinity antibodies in response to antigen challenge.
Related Conjugates and Formulations
Conjucates
Unconjugated
Antigen Details
Immunogen
A synthetic peptide: Human CD19
Antigen Species
Human
Gene ID
930
Uniprot ID
Biology Area
Immunology, Stem cells
Chemical Properties
Stability & Storage
Stability & StorageStore at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc

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