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CLEC12A/MICL/CLL-1 Protein, Human, Recombinant (hFc & Avi), Biotinylated

(Synonyms: PRO34150, MICL, HECL, DLEC, DCAL-2, DCAL2, CLL-1, CLL1, CLECSF7, CLECSF11, CLEC12A, CD371, CD303) Copy Product Info

Synonyms: PRO34150, MICL, HECL, DLEC, DCAL-2, DCAL2, CLL-1, CLL1, CLECSF7, CLECSF11, CLEC12A, CD371, CD303

Catalog No. TMPK-01002 Copy Product Info
CLEC12A has recently been identified as an antigen, expressed on leukemic stem cells and leukemic blasts. Given the fact that this expression profile seems stable throughout diagnosis, treatment and relapse on leukemic blasts and leukemic stem cells, CLEC12A can be considered a highly potent and reliable marker for the detection of measurable residual disease and therefore applicable for risk stratification and prognostication in AML. CLEC12A/MICL/CLL-1 Protein, Human, Recombinant (hFc & Avi), Biotinylated is expressed in HEK293 mammalian cells with N-hFc-Avi tag. The predicted molecular weight is 51.7 kDa and the accession number is Q5QGZ9-2.

Product Introduction

Bioactivity
Bioactivity
Immobilized Anti-CLEC12A Antibody, hFc Tag at 1μg/ml (100μl/Well) on the plate. Dose response curve for Biotinylated Human CLEC12A, hFc Tag with the EC50 of 14.9ng/ml determined by ELISA.
Description
CLEC12A has recently been identified as an antigen, expressed on leukemic stem cells and leukemic blasts. Given the fact that this expression profile seems stable throughout diagnosis, treatment and relapse on leukemic blasts and leukemic stem cells, CLEC12A can be considered a highly potent and reliable marker for the detection of measurable residual disease and therefore applicable for risk stratification and prognostication in AML. CLEC12A/MICL/CLL-1 Protein, Human, Recombinant (hFc & Avi), Biotinylated is expressed in HEK293 mammalian cells with N-hFc-Avi tag. The predicted molecular weight is 51.7 kDa and the accession number is Q5QGZ9-2.
Species
Human
Expression System
HEK293 Cells
TagN-hFc-Avi
Accession NumberQ5QGZ9-2
ConstructionHis65-Ala265
Protein Purity
> 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC
Endotoxin< 1.0 EU/μg of the protein as determined by the LAL method.
FormulationLyophilized from a solution filtered through a 0.22 μm filter, containing PBS (pH 7.4). Typically, 8% trehalose is incorporated as a protective agent before lyophilization.
ReconstitutionReconstitute the lyophilized protein in distilled water. The product concentration should not be less than 100 μg/ml. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing.
Stability & StorageIt is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
ShippingIn general, lyophilized powders are shipped with blue ice, while solutions are shipped with dry ice.
SynonymsPRO34150, MICL, HECL, DLEC, DCAL-2, DCAL2, CLL-1, CLL1, CLECSF7, CLECSF11, CLEC12A, CD371, CD303
Research Background
Chemical Properties
Molecular Weight51.7 kDa (predicted). Due to glycosylation, the protein migrates to 67-70 kDa based on Tris-Bis PAGE result.
Storage & Solubility Information
StorageLyophilized powder: -20~-80°C for 1 year | Solution: -80°C for 6 months

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Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc

Keywords

Related Tags: CLEC12A/MICL/CLL-1 Protein, Human, Recombinant (hFc & Avi), Biotinylated chemical structure | CLEC12A/MICL/CLL-1 Protein, Human, Recombinant (hFc & Avi), Biotinylated molecular weight