Immobilized Human CD27, His Tag at 0.5 μg/ml (100 μl/well) on the plate. Dose response curve for Human CD27 Ligand, hFc Tag with the EC50 of 0.14 μg/ml determined by ELISA.

CD27/TNFRSF7 Protein, Human, Recombinant (HEK293, His) is expressed in HEK293 Cells. The accession number is P26842.

Human

HEK293 Cells

Tp55,TNFRSF7,T14,S152. LPFS2,S152

Thr21-Ile192

> 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC

Reconstitute the lyophilized protein in distilled water. The product concentration should not be less than 100 μg/ml. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing.

It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.

CD27, also known as TNFRSF7, is an approximately 55 kDa transmembrane protein in the TNF receptor superfamily. It functions as a co‑stimulatory molecule that supports lymphocyte activation and survival. It binds to ligand CD70, and plays a key role in regulating B-cell activation and immunoglobulin synthesis.