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Anti-LAMP2 Antibody (6M137) is a Mouse antibody targeting LAMP2. Anti-LAMP2 Antibody (6M137) can be used in WB,ELISA,ICC/IF.
Pack Size | Price | Availability | Quantity |
---|---|---|---|
100 μL | $198 | 7-10 days |
Description | Anti-LAMP2 Antibody (6M137) is a Mouse antibody targeting LAMP2. Anti-LAMP2 Antibody (6M137) can be used in WB,ELISA,ICC/IF. |
Alias | lysosomal-associated membrane protein 2, LGP110, LAMPB, LAMP-2, CD107b |
Ig Type | Monoclonal Mouse IgG1 |
Clone | 6M137 |
Reactivity | Human |
Verified Activity | 1. Anti-LAMP2 mouse monoclonal antibody at 1:500 dilution. -Lane A: HepG2 Whole Cell Lysate. -Lane B: Jurkat Whole Cell Lysate. -Lane C: Hela Whole Cell lysate. -Lysates/proteins at 30 μg per lane. -Secondary -Goat Anti-Mouse IgG (H+L)/HRP at 1/10000 dilution. -Developed using the ECL technique. -Performed under reducing conditions. -Predicted band size:45 kDa. 2. Immunofluorescence staining of LAMP2 in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS,blocked with 10% serum, and incubated with mouse anti-human LAMP2 monoclonal antibody (dilution ratio 1:60) at 4°C overnight. Then cells were stained with the Alexa Fluor®488-conjugated Goat Anti-mouse IgG secondary antibody (green). Positive staining was localized to Cytoplasm. |
Application | WB,ELISA,ICC/IF |
Recommended Dose | WB: 1:500-1:2000; ELISA: 1:1000-1:2000; ICC-IF: 1:20-1:100 |
Antibody Type | Monoclonal |
Host Species | Mouse |
Construction | This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human LAMP2/CD107b (rh LAMP2/CD107b; TMPY-04115; NP_054701.1; Met1-Ile375). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography. |
Purification | Protein A |
Appearance | Liquid |
Formulation | 0.2 μm filtered solution in PBS |
Research Background | LAMP2 (Lysosomal Associated Membrane Protein 2) is a Protein Coding gene. LAMP2, also known as CD107b (Cluster of Differentiation 107b), is a member of a family of membrane glycoproteins. This glycoprotein provides selectins with carbohydrate ligands. It may play a role in tumor cell metastasis. It may also function in the protection, maintenance, and adhesion of the lysosome. In humans, LAMP2, the causative gene of Danon disease, located on chromosome Xq24, encodes the lysosome-associated membrane protein-2 (LAMP-2). LAMP-2 deficiency, or Danon disease, is a rare X-linked lysosomal disease characterized by cardiomyopathy, vacuolar myopathy, and mental retardation. LAMP2 cardiomyopathy is an X-linked and highly progressive myocardial storage disorder associated with diminished survival, which clinically resembles sarcomeric hypertrophic cardiomyopathy. |
Conjucates | Unconjugated |
Immunogen | Recombinant Human LAMP2/CD107b Protein (TMPY-04115) |
Antigen Species | Human |
Stability & Storage | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free. |
Transport | Shipping with blue ice. |
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