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Anti-CD97 Antibody (4G322)
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Catalog No. TMAY-01823
Alias TM7LN1, CD97, adhesion G protein-coupled receptor E5
Anti-CD97 Antibody (4G322) is a Mouse antibody targeting CD97. Anti-CD97 Antibody (4G322) can be used in ELISA,ELISA(Cap),ICC/IF.
Anti-CD97 Antibody (4G322)
Anti-CD97 Antibody (4G322)
😃Good
Catalog No. TMAY-01823Alias TM7LN1, CD97, adhesion G protein-coupled receptor E5
Anti-CD97 Antibody (4G322) is a Mouse antibody targeting CD97. Anti-CD97 Antibody (4G322) can be used in ELISA,ELISA(Cap),ICC/IF.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 20 μL | $69 | 7-10 days | |
| 100 μL | $199 | 7-10 days |
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Chemical Properties
| Description | Anti-CD97 Antibody (4G322) is a Mouse antibody targeting CD97. Anti-CD97 Antibody (4G322) can be used in ELISA,ELISA(Cap),ICC/IF. |
| Alias | TM7LN1, CD97, adhesion G protein-coupled receptor E5 |
| Ig Type | Monoclonal Mouse IgG1 |
| Clone | 4G322 |
| Reactivity | Human |
| Verified Activity | Immunofluorescence staining of Human CD97 in Hela cells. Cells were fixed with 4% PFA, blocked with 10% serum, and incubated with Mouse anti-Human CD97 monoclonal antibody (1:60) at 37°C 1 hour. Then cells were stained with the Alexa Fluor® 488-conjugated Goat Anti-mouse IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to cells membranes. |
| Application | ELISA,ELISA(Cap),ICC/IF |
| Recommended Dose | ELISA: 1:1000-1:2000; ICC-IF: 1:20-1:100; ELISA(Cap): 1:250-1:2000 |
| Antibody Type | Monoclonal |
| Host Species | Mouse |
| Construction | This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human CD97 (rh CD97; TMPY-01365; NP_001775.2; Met1-Gln398). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography. |
| Purification | Protein A |
| Appearance | Liquid |
| Formulation | 0.2 μm filtered solution in PBS |
| Research Background | The cluster of differentiation (CD) system is commonly used as cell markers in immunophenotyping. Different kinds of cells in the immune system can be identified through the surface CD molecules which associating with the immune function of the cell. There are more than 32 CD unique clusters and subclusters have been identified. Some of the CD molecules serve as receptors or ligands important to the cell through initiating a signal cascade which then alter the behavior of the cell. Some CD proteins do not take part in cell signal process but have other functions such as cell adhesion. The CD97 is a receptor predominantly expressed in leukocytes and belongs to a new group of seven-span transmembrane molecules, which is also designed EGF-TM7 family. The family members are characterized by an extended extracellular region with several N-terminal epidermal growth factor-like domains two of which contain a calcium-binding site. Mature CD 97 has two noncovalently associated subunits and is composed of a large extracellular protein (CD97 alpha) and a seven-membrane spanning protein (CD97 beta). CD97 is considered as a defining feature of G protein-coupled receptors. The effects that lymphocytes and erythrocytes adhere to CD97-transfected COS cells suggest that CD97 has the ability to bind cellular ligands. CD97 alpha has three alternatively spliced isoforms that are related to the calcium-binding EGF-like repeats in the microfibril protein fibrillin. Leukocytes strongly positive for CD97 are concentrated at sites of inflammation relative to CD97 expression in normal lymphoid tissues. |
| Conjucates | Unconjugated |
| Immunogen | Recombinant Human CD97 protein (TMPY-01365) |
| Antigen Species | Human |
Stability & Storage
| Stability & Storage | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free. |
| Transport | Shipping with blue ice. |
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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