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Anti-CD3 zeta/CD247 Antibody (5W525)
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Catalog No. TMAH-00183
Alias TCRZ, T3Z, IMD25, CD3-ζ, CD3-ZETA, CD3Z, CD3Q, CD3H, CD3 ζ/CD247, CD247 molecule
Anti-CD3 zeta/CD247 Antibody (5W525) is an antibody targeting CD3 zeta/CD247. Anti-CD3 zeta/CD247 Antibody (5W525) can be used in ELISA, FCM.
Anti-CD3 zeta/CD247 Antibody (5W525)
Anti-CD3 zeta/CD247 Antibody (5W525)
😃Good
Catalog No. TMAH-00183Alias TCRZ, T3Z, IMD25, CD3-ζ, CD3-ZETA, CD3Z, CD3Q, CD3H, CD3 ζ/CD247, CD247 molecule
Anti-CD3 zeta/CD247 Antibody (5W525) is an antibody targeting CD3 zeta/CD247. Anti-CD3 zeta/CD247 Antibody (5W525) can be used in ELISA, FCM.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 50 μL | $208 | 7-10 days | |
| 100 μL | $349 | 7-10 days |
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Product Introduction
Bioactivity
Related Conjugates and Formulations
Antigen Details
Chemical Properties
| Description | Antibody Type: Recombinant Rabbit Monoclonal Application: ELISA, FCM Reactivity: Human |
| Alias | TCRZ, T3Z, IMD25, CD3-ζ, CD3-ZETA, CD3Z, CD3Q, CD3H, CD3 ζ/CD247, CD247 molecule |
| Ig Type | Rabbit IgG |
| Clone | 5W525 |
| Reactivity | Human |
| Verified Activity | Overlay Peak curve showing Jurkat cells surface stained with TMAH-00183 (red line) at 1:50. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1µg/1*10^6 cells) for 45min at 4°C. The secondary antibody used was FITC-conjugated Goat Anti-rabbit IgG(H+L) at 1:200 dilution for 35min at 4°C.Control antibody (green line) was rabbit IgG (1µg/1*10^6 cells) used under the same conditions. Acquisition of >10,000 events was performed. |
| Application | ELISA, FCM |
| Recommended Dose | FCM:1:50-1:200. |
| Antibody Type | Monoclonal |
| Subcellular Localization | Cell membrane; Single-pass type I membrane protein. |
| Construction | Recombinant Antibody |
| Purification | Affinity-chromatography |
| Appearance | Liquid |
| Formulation | Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| Research Background | Part of the TCR-CD3 complex present on T-lymphocyte cell surface that plays an essential role in adaptive immune response. When antigen presenting cells (APCs) activate T-cell receptor (TCR), TCR-mediated signals are transmitted across the cell membrane by the CD3 chains CD3D, CD3E, CD3G and CD3Z. All CD3 chains contain immunoreceptor tyrosine-based activation motifs (ITAMs) in their cytoplasmic domain. Upon TCR engagement, these motifs become phosphorylated by Src family protein tyrosine kinases LCK and FYN, resulting in the activation of downstream signaling pathways. CD3Z ITAMs phosphorylation creates multiple docking sites for the protein kinase ZAP70 leading to ZAP70 phosphorylation and its conversion into a catalytically active enzyme. Plays an important role in intrathymic T-cell differentiation. Additionally, participates in the activity-dependent synapse formation of retinal ganglion cells (RGCs) in both the retina and dorsal lateral geniculate nucleus (dLGN). |
| Conjucates | Unconjugated |
| Immunogen | A synthetic peptide: Human CD247 |
| Antigen Species | Human |
| Gene ID | 919 |
| Uniprot ID | |
| Biology Area | Immunology, Stem cells |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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