Measured by its binding ability in a functional ELISA. Immobilized canine NRG1 at 10 μg/ml (100 μl/well) can bind human ErbB4-Fc , The EC50 of human ErbB4-Fc is 0.19-0.43 μg/ml.

Neuregulin-1/NRG1 Protein, Canine, Recombinant is expressed in HEK293 mammalian cells. The predicted molecular weight is 24.7 kDa and the accession number is XP_858147.1.

Canine

HEK293 Cells

neuregulin 1

A DNA sequence encoding the canine NRG1 (XP_858147.1) extracellular domain (Ser19-Lys240) was expressed with five amino acids (DDDDK) at the C- terminus. Predicted N terminal: Ser 19

> 95 % as determined by SDS-PAGE

A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.

It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.

Neuregulin 1 or NRG1 is one of four proteins in the neuregulin family that act on the EGFR family of receptors. This growth factor was originally identified as a 44-kD glycoprotein that interacts with the NEU / ERBB2 receptor tyrosine kinase to increase its phosphorylation on tyrosine residues. NRG1 is a trophic factor that has been implicated in neural development, neurotransmission, and synaptic plasticity. NRG1 has multiple isoforms that are generated by the usage of different promoters and alternative splicing of a single gene. Neuregulin 1 (NRG1) is essential for the development and function of multiple organ systems, and its dysregulation has been linked to diseases such as cancer and schizophrenia. NRG1 is a schizophrenia candidate gene and plays an important role in brain development and neural function. Schizophrenia is a complex disorder, with etiology likely due to epistasis.