SPARC Protein, Human, Recombinant (His)

Catalog No. TMPY-01182 Copy Product Info

SPARC Protein, Human, Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 33.8 kDa and the accession number is P09486.

SPARC Protein, Human, Recombinant (His)

Catalog No. TMPY-01182

Copy Product Info

SPARC Protein, Human, Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 33.8 kDa and the accession number is P09486.

Pack Size	Price	USA Stock	Global Stock
5 μg	$68	7-10 days	7-10 days
10 μg	$108	7-10 days	7-10 days
20 μg	$178	7-10 days	7-10 days
50 μg	$359	7-10 days	7-10 days
100 μg	$696	7-10 days	7-10 days
200 μg	$1,060	7-10 days	7-10 days
500 μg	$2,230	7-10 days	7-10 days
1 mg	$4,530	7-10 days	7-10 days

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For In stock only · Estimated delivery:USA Stock (1-2 days) Global Stock (5-7 days)

For research use only—not for human use. No sales to individuals. Use as intended only.

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Product Information

Biological Activity	Immobilized Human SPARC, His Tag at 0.5 μg/ml (100 μl/well) on the plate. Dose response curve for Anti-SPARC Antibody, hFc Tag with the EC50 of 0.10 μg/ml determined by ELISA.
Description	SPARC Protein, Human, Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 33.8 kDa and the accession number is P09486.
Species	Human
Expression System	HEK293 Cells
Tag	C-His
Accession Number	P09486
Synonyms	SPARC,secreted protein, acidic, cysteine-rich (osteonectin),ON,BM-40
Construction	Ala18-Ile303
Protein Purity	> 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC
Molecular Weight	33.8 kDa (Predicted); 40-50 kDa (Due to glycosylation)
Endotoxin	< 1.0 EU/μg of the protein as determined by the LAL method.
Formulation	Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Reconstitute the lyophilized protein in distilled water. The product concentration should not be less than 100 μg/ml. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing.
Stability & Storage	It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
Shipping	In general, lyophilized powders are shipped with blue ice, while solutions are shipped with dry ice.
Research Background	Secreted protein acidic and rich in cysteine (SPARC/osteonectin/BM40) is one of the most abundant non-collagenous protein expressed in mineralized tissues.The capacity of SPARC to influence pathways involved in extracellular matrix assembly such as procollagen processing and collagen fibril formation as well as the capacity to influence osteoblast differentiation and osteoclast activity will be addressed.