Shopping Cart
  • Remove All
  • TargetMol
    Your shopping cart is currently empty

SPARC Protein, Human, Recombinant (His)

TargetMol | SPR
Catalog No. TMPY-01182

SPARC Protein, Human, Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 33.8 kDa and the accession number is P09486.

SPARC Protein, Human, Recombinant (His)

SPARC Protein, Human, Recombinant (His)

TargetMol | SPR
Catalog No. TMPY-01182
SPARC Protein, Human, Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 33.8 kDa and the accession number is P09486.
Pack SizePriceAvailabilityQuantity
100 μg$6007-10 days
1 mg$3,9107-10 days
Bulk & Custom
Add to Cart
All TargetMol products are for research purposes only and cannot be used for human consumption. We do not provide products or services to individuals. Please comply with the intended use and do not use TargetMol products for any other purpose.
Questions
View More

Product Information

Biological Activity
Immobilized Human SPARC, His Tag at 0.5 μg/ml (100 μl/well) on the plate. Dose response curve for Anti-SPARC Antibody, hFc Tag with the EC50 of 0.10 μg/ml determined by ELISA.
Description
SPARC Protein, Human, Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 33.8 kDa and the accession number is P09486.
Species
Human
Expression System
HEK293 Cells
TagC-His
Accession NumberP09486
Synonyms
SPARC,secreted protein, acidic, cysteine-rich (osteonectin),ON,BM-40
Construction
Ala18-Ile303
Protein Purity
> 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC
Molecular Weight33.8 kDa (Predicted); 40-50 kDa (Due to glycosylation)
EndotoxinLess than 1EU per μg by the LAL method.
FormulationLyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
Reconstitute the lyophilized protein in distilled water. The product concentration should not be less than 100 μg/ml. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing.
Stability & Storage
It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
ShippingIn general, Lyophilized powders are shipping with blue ice. Solutions are shipping with dry ice.
Research Background
Secreted protein acidic and rich in cysteine (SPARC/osteonectin/BM40) is one of the most abundant non-collagenous protein expressed in mineralized tissues.The capacity of SPARC to influence pathways involved in extracellular matrix assembly such as procollagen processing and collagen fibril formation as well as the capacity to influence osteoblast differentiation and osteoclast activity will be addressed.

Dose Conversion

You can also refer to dose conversion for different animals. More

Sci Citations

Calculator

  • Reconstitution Calculator
  • Recombinant Protein Dilution Calculator
  • Specific Activity Calculator

Tech Support

Please read the User Guide of Recombinant Proteins for more specific information.

Keywords