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Anti-BANF1 Antibody (7K215)

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Catalog No. TMAB-03023

Anti-BANF1 Antibody (7K215) is a Rabbit antibody targeting BANF1. Anti-BANF1 Antibody (7K215) can be used in IHC-P,IHC-Fr,ICC/IF,IF,ELISA.

Anti-BANF1 Antibody (7K215)

Anti-BANF1 Antibody (7K215)

😃Good
Catalog No. TMAB-03023
Anti-BANF1 Antibody (7K215) is a Rabbit antibody targeting BANF1. Anti-BANF1 Antibody (7K215) can be used in IHC-P,IHC-Fr,ICC/IF,IF,ELISA.
Pack SizePriceUSA WarehouseGlobal WarehouseQuantity
50 μL$2627-10 days7-10 days
100 μL$4737-10 days7-10 days
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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]
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Product Introduction

Bioactivity
Description
Anti-BANF1 Antibody (7K215) is a Rabbit antibody targeting BANF1. Anti-BANF1 Antibody (7K215) can be used in IHC-P,IHC-Fr,ICC/IF,IF,ELISA.
Ig Type
IgG
Clone
7K215
Reactivity
Human,Mouse,Rat
Application
Recommended Dose
IHC-P: 1:50-200; IHC-Fr: 1:50-200; ICC/IF: 1:50-200; IF: 1:50-200; ELISA: 1:5000-10000
Antibody Type
Monoclonal
Host SpeciesRabbit
Subcellular LocalizationNucleus. Cytoplasm. Chromosome. Significantly enriched at the nuclear inner membrane, diffusely throughout the nucleus during interphase and concentrated at the chromosomes during the M-phase. May be included in HIV-1 virions via its interaction with viral GAG polyprotein. Tissue Specificity : Widely expressed. Expressed in colon, brain, heart, kidney, liver, lung, ovary, pancreas, placenta, prostate, skeletal muscle, small intestine, spleen and testis. Not detected in thymus and peripheral blood leukocytes.
Tissue SpecificityWidely expressed. Expressed in colon, brain, heart, kidney, liver, lung, ovary, pancreas, placenta, prostate, skeletal muscle, small intestine, spleen and testis. Not detected in thymus and peripheral blood leukocytes.
ConstructionRecombinant Antibody
AppearanceLiquid
Formulation0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Concentration1mg/ml
Research BackgroundBarrier-to-autointegration factor (BAF) binds non-specifically to double stranded DNA, possibly to play a role in tissue- or cell type-specific gene expression by interacting with different homeodomain transcription factors. BAF compresses chromatin structure and interacts with the LEM domain of nuclear proteins to play a crucial role in membrane recruitment and chromatin decondensation during nuclear assembly. Additionally, retroviruses like HIV-1 incorporate BAF from host cells into preintegration complexes (PICs) to prevent autointegration of retroviral DNA and thereby promote integration of retroviral DNA into the host chromosome.
Antigen Details
Immunogen
Recombinant Protein: human BANF1
Antigen Species
Human
Gene Name
BANF1
Gene ID
Protein Name
Barrier-to-autointegration factor
Uniprot ID
Function
Plays fundamental roles in nuclear assembly, chromatin organization, gene expression and gonad development. May potently compress chromatin structure and be involved in membrane recruitment and chromatin decondensation during nuclear assembly. Contains 2 non-specific dsDNA-binding sites which may promote DNA cross-bridging. Exploited by retroviruses for inhibiting self-destructing autointegration of retroviral DNA, thereby promoting integration of viral DNA into the host chromosome. EMD and BAF are cooperative cofactors of HIV-1 infection. Association of EMD with the viral DNA requires the presence of BAF and viral integrase. The association of viral DNA with chromatin requires the presence of BAF and EMD.
Chemical Properties
Molecular WeightTheoretical: 10 kDa.
Stability & Storage
Stability & StorageStore at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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