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c-Kit Protein, Human, Recombinant (hFc)

Catalog No. TMPY-04051
Synonyms: SCFR, v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, PBT, CD117, C-Kit

c-Kit Protein, Human, Recombinant (hFc) is expressed in HEK293 mammalian cells with hFc tag. The predicted molecular weight is 82 kDa and the accession number is P10721-2.

All products from TargetMol are for Research Use Only. Not for Human or Veterinary or Therapeutic Use.
c-Kit Protein, Human, Recombinant (hFc)
Pack Size Availability Price/USD Quantity
50 μg In stock $ 255.00
100 μg 5 days $ 435.00
200 μg 5 days $ 741.00
500 μg 5 days $ 1,490.00
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Biological Description
Technical Params
Product Properties
References and Literature
Biological Information Immobilized Human SCF His at 2 μg/ml (100 μl/well) can bind Human c-Kit hFc , the EC50 of Human c-Kit hFc is 20-60 ng/mL.

Description c-Kit Protein, Human, Recombinant (hFc) is expressed in HEK293 mammalian cells with hFc tag. The predicted molecular weight is 82 kDa and the accession number is P10721-2.
Species Human
Expression System HEK293 Cells
Tag C-hFc
Accession Number P10721-2
Synonyms SCFR, v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, PBT, CD117, C-Kit
Construction The extracellular domain of Human KIT (P10721-2) (Met1-Thr516) was expressed with the Fc region of Human IgG1 at the C-terminus.
Protein Purity > 95 % as determined by SDS-PAGE

Molecular Weight 82 kDa (predicted)
Endotoxin < 1.0 EU/μg of the protein as determined by the LAL method.
Formulation Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH 7.4. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
Reconstitution A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.
Stability & Storage

It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at-80℃. For reconstituted protein solutions, the solution can be stored at -20°c to -80'c for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.

Shipping

In general, Lyophilized powders are shipping with blue ice.

Research Background C-Kit is a type 3 transmembrane receptor for MGF (mast cell growth factor, also known as stem cell factor). c-Kit contains 5 Ig-like C2-type (immunoglobulin-like) domains and 1 protein kinase domain. It belongs to the protein kinase superfamily, tyr protein kinase family, and CSF-1/PDGF receptor subfamily. C-Kit has tyrosine-protein kinase activity. Binding of the ligands leads to the autophosphorylation of KIT and its association with substrates such as phosphatidylinositol 3-kinase. Antibodies to c-Kit are widely used in immunohistochemistry to help distinguish particular types of tumor in histological tissue sections. It is used primarily in the diagnosis of GISTs. In GISTs, c-Kit staining is typically cytoplasmic, with stronger accentuation along the cell membranes. C-Kit antibodies can also be used in the diagnosis of mast cell tumors and in distinguishing seminomas from embryonal carcinomas. Mutations in the c-Kit gene are associated with gastrointestinal stromal tumors, mast cell disease, acute myelogenous leukemia, and piebaldism. Defects in KIT are a cause of acute myelogenous leukemia (AML). AML is a malignant disease in which hematopoietic precursors are arrested in an early stage of development. Note=Somatic mutations that lead to constitutive activation of KIT are detected in AML patients.Cancer ImmunotherapyImmune CheckpointImmunotherapyTargeted Therapy

References and Literature

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Keywords

c-Kit Protein, Human, Recombinant (hFc) SCFR CD 117 v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog PBT CD117 CD-117 C-Kit recombinant recombinant-proteins proteins protein

 

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