CD28 Protein, Human/Cynomolgus, Recombinant (His)

T-cell-specific surface glycoprotein CD28(CD28) is a single-pass typeI membrane protein which contains one Ig-likeV-type (immunoglobulin-like) domain. It belongs to the immunoglobulin(Ig) superfamily. CD28 is one of the molecules expressed on T cells that provide co-stimulatory signals, which are required for T cell activation.CD28 co-stimulation is necessary for CD4 positive T-cell proliferation and survival, interleukin-2 production, and T-helper type-2 development. Human post-thymic regulatory T cells require CD28 co-stimulation to expand and maintain potent suppressive function in vivo. Apoptosis plays a key role in the age-related decline of CD28 expression and in immunosenescence. CD28 is the receptor for CD80 (B7.1) and CD86 (B7.2). When activated by Toll-like receptor ligands, the CD80 expression is upregulated in antigen presenting cells (APCs). The CD86 expression on antigen presenting cells is constitutive. CD28 is the only B7 receptor constitutively expressed on naive T cells.

Pack Size	Availability	Price/USD
10 μg	In stock	$ 56.00
50 μg	5 days	$ 169.00
500 μg	5 days	$ 1,310.00
1 mg	5 days	$ 3,060.00

Pack Size

Availability

Price/USD

Quantity

10 μg

In stock

$ 56.00

50 μg

5 days

$ 169.00

500 μg

5 days

$ 1,310.00

1 mg

5 days

$ 3,060.00

Biological Information

1. Loaded Anti-Human CD28 mAb-Fc on Protein A Biosensor, can bind Human/Cynomolgus CD28-His with an affinity constant of 0.77 nM as determined in BLI assay. (Regularly tested) 2. Immobilized Human/Cynomolgus CD28-His at 2μg/ml (100 μl/well) can bind Anti-CD28 mAb The ED50 of Anti-CD28 mAb is 15.3 ng/ml. (Regularly tested)

Description

Species	Human,Cynomolgus
Expression System	Human Cells
Tag	N-6His
Accession Number	P10747
Synonyms	CD28 antigen, CD28 molecule, T-cell-specific surface glycoprotein CD28, CD28, Tp44
Amino Acid	Asn19-Pro152
Construction	Recombinant Human T-cell-specific Surface Glycoprotein CD28 is produced by our Mammalian expression system and the target gene encoding Asn19-Pro152 is expressed with a 6His tag at the N-terminus.
Protein Purity	Greater than 95% as determined by reducing SDS-PAGE. (QC verified)
Molecular Weight	30-40 KDa, reducing conditions

Endotoxin	Less than 0.1 ng/µg (1 EU/µg) as determined by LAL test.
Formulation	Lyophilized from a 0.2 μm filtered solution of 20mM Tris-HCl, 150mM NaCl, pH 8.0.
Reconstitution	Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
Stability & Storage	Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature listed below.
Research Background	T-cell-specific surface glycoprotein CD28(CD28) is a single-pass typeI membrane protein which contains one Ig-likeV-type (immunoglobulin-like) domain. It belongs to the immunoglobulin(Ig) superfamily. CD28 is one of the molecules expressed on T cells that provide co-stimulatory signals, which are required for T cell activation.CD28 co-stimulation is necessary for CD4 positive T-cell proliferation and survival, interleukin-2 production, and T-helper type-2 development. Human post-thymic regulatory T cells require CD28 co-stimulation to expand and maintain potent suppressive function in vivo. Apoptosis plays a key role in the age-related decline of CD28 expression and in immunosenescence. CD28 is the receptor for CD80 (B7.1) and CD86 (B7.2). When activated by Toll-like receptor ligands, the CD80 expression is upregulated in antigen presenting cells (APCs). The CD86 expression on antigen presenting cells is constitutive. CD28 is the only B7 receptor constitutively expressed on naive T cells.

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CD28 Protein, Human/Cynomolgus, Recombinant (His)

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