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Anti-CD50 Antibody (5P32) is a Mouse antibody targeting CD50. Anti-CD50 Antibody (5P32) can be used in ELISA(Cap),FCM.
Pack Size | Price | Availability | Quantity |
---|---|---|---|
20 μL | $69 | 7-10 days | |
100 μL | $198 | 7-10 days |
Description | Anti-CD50 Antibody (5P32) is a Mouse antibody targeting CD50. Anti-CD50 Antibody (5P32) can be used in ELISA(Cap),FCM. |
Alias | intercellular adhesion molecule 3, ICAM-R, ICAM-3, CDW50, CD50 |
Ig Type | Monoclonal Mouse IgG2a |
Clone | 5P32 |
Reactivity | Human |
Verified Activity | Flow cytometric analysis of human ICAM3(CD50) expression on human peripheral blood lymphocytes. Cells were stained with purified anti-Human ICAM3(CD50), then a FITC-conjugated second step antibody. The histogram were derived from events with the forward and side light-scatter characteristics of viable lymphocytes. |
Application | ELISA(Cap),FCM |
Recommended Dose | FCM: 1:25-1:100; ELISA(Cap): 1:250-1:2000 |
Antibody Type | Monoclonal |
Host Species | Mouse |
Construction | This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human CD50 / ICAM-3 (rh CD50 / ICAM-3; TMPY-01430; CAA49369.1; Met1-His485). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography. |
Purification | Protein A |
Appearance | Liquid |
Formulation | 0.2 μm filtered solution in PBS |
Research Background | The protein ICAM-3, also known as CD50, is a member of the intercellular adhesion molecule (ICAM) family consisting of three members. It is a DC-SIGN ligand that is constitutively expressed on resting leukocytes and is thus an important molecule for the first immune response. ICAM-3 comprises five immunoglobulin-like domains and binds LFA-1 through its two N-terminal domains. It functions not only as an adhesion molecule but also as a potent signaling molecule. ICAM-3 binds to LFA-1 on antigen-presenting cells (APC) stabilizing the T cell-APC interaction, facilitating signaling through the CD3/TCR complex. However, recent evidence using cultured and transformed T cells suggests ICAM-3 may also function in signaling. It has been reported that the CD50 molecule can play a role in developing functionally mature T lymphocytes and its expression increases during the maturation process of T lymphocytes. Also, the interactions of ICAM-3 and LFA-1 facilitate HIV-1- induced virological synapse formation between T cells. ICAM-3 is associated with an increase in cellular radio-resistance and cancer cell proliferation. It could be considered as a candidate for anti-cancer drug development and as a cancer diagnostic marker. |
Conjucates | Unconjugated |
Others Formats | APC/FITC/PE |
Antibody Types Available | 4 |
Immunogen | Recombinant Human CD50 / ICAM-3 protein (TMPY-01430) |
Antigen Species | Human |
Stability & Storage | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free. |
Transport | Shipping with blue ice. |
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