Immobilized Human CD34, His Tag at 0.5 μg/ml (100 μl/Well) on the plate. Dose response curve for Anti-CD34 Antibody, hFc Tag with the EC50 of 19.9 ng/ml determined by ELISA.

CD34 Protein, Human, Recombinant (His) V2 is expressed in HEK293 Cells. The accession number is P28906-1.

Human

HEK293 Cells

CD34 molecule

Ser32-Thr290

> 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC

Reconstitute the lyophilized protein in sterile deionized water. The product concentration should not be less than 100 μg/mL. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing.

Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.

CD34 is a transmembrane phosphoglycoprotein, first identified on hematopoietic stem and progenitor cells. Clinically, it is associated with the selection and enrichment of hematopoietic stem cells for bone marrow transplants. Due to these historical and clinical associations, CD34 expression is almost ubiquitously related to hematopoietic cells, and it is a common misconception that CD34-positive (CD34()) cells in nonhematopoietic samples represent hematopoietic contamination.