1. Immobilized Mouse TNFSF15, His Tag at 0.5μg/ml (100μl/well) on the plate. Dose response curve for Anti-TNFSF15 Antibody, hFc Tag with the EC50 of 11.6ng/ml determined by ELISA (QC Test). 2. Mouse DR3, His Tag immobilized on CM5 Chip can bind Mouse TNFSF15, His Tag with an affinity constant of 0.96 nM as determined in SPR assay.

TNFSF15 Protein, Mouse, Recombinant (His & Avi) is expressed in HEK293 mammalian cells with N-His-Avi tag. The predicted molecular weight is 24.40 kDa and the accession number is Q5UBV8.

Mouse

HEK293 Cells

VEGI-251,VEGI192A,VEGI,TNFSF15,TL1A,TL1

Ala61-Leu252

> 90% as determined by Tris-Bis PAGE; > 95% as determined by HPLC

Reconstitute the lyophilized protein in distilled water. The product concentration should not be less than 100 μg/ml. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing.

It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.

TL1A and its functional receptor DR3 are members of the TNF/TNFR superfamilies of proteins.TL1A and DR3 are abundantly localized at inflamed intestinal areas of patients with IBD and mice with experimental ileitis or colitis and actively participate in the immunological pathways that underlie mucosal homeostasis and intestinal inflammation.Recently, an important role was demonstrated for TL1A/DR3 as potential mediators of intestinal fibrosis that is associated with the presence of gut inflammation.