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Anti-His Tag Antibody (3U153)
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Catalog No. TMAY-00956
Anti-His Tag Antibody (3U153) is a Mouse antibody targeting His Tag. Anti-His Tag Antibody (3U153) can be used in ELISA.
Anti-His Tag Antibody (3U153)
Anti-His Tag Antibody (3U153)
😃Good
Catalog No. TMAY-00956
Anti-His Tag Antibody (3U153) is a Mouse antibody targeting His Tag. Anti-His Tag Antibody (3U153) can be used in ELISA.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 20 μL | $69 | 7-10 days | |
| 100 μL | $235 | 7-10 days | |
| 500 μL | $587 | 7-10 days |
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Product Introduction
Bioactivity
Related Conjugates and Formulations
Antigen Details
Chemical Properties
| Description | Antibody Type: Recombinant Mouse Monoclonal Application: ELISA Reactivity: other |
| Ig Type | Mouse IgG2b |
| Clone | 3U153 |
| Reactivity | other |
| Application | ELISA |
| Recommended Dose | ELISA: 1:5000-1:20000 |
| Antibody Type | Monoclonal |
| Host Species | Mouse |
| Construction | This product is a recombinant monoclonal antibody expressed from HEK293 cells. |
| Purification | Protein A |
| Appearance | Liquid |
| Formulation | 0.2 μm filtered solution in PBS |
| Research Background | The hexahistidine tag (6× His-tag) is the most frequently used affinity tag for protein enrichment. His-tagged proteins can be purified easily by the chelated metal ions as affinity ligands. The basis for affinity purification is known as immobilized metal affinity chromatography (IMAC). His-tag can bind best to IMAC resin in near-neutral buffer conditions (physiologic pH and ionic strength), and thus the fusion proteins can be eluted with binding buffer containing certain concentrations of imidazole. If possible, the elution is also accomplished with low pH (e.g., 0.1 M glycine-HCl, pH 2.5) or an excess of strong chelator (e.g., EDTA). |
| Conjucates | Unconjugated |
| Others Formats | HRP |
| Antibody Types Available | 2 |
| Immunogen | A synthetic peptide: 6×His |
| Antigen Species | other |
Stability & Storage
| Stability & Storage | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free. |
| Transport | Shipping with blue ice. |
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For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . 
Tech Support
Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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