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Anti-GOLM1 Antibody (1M115) is an antibody targeting GOLM1. Anti-GOLM1 Antibody (1M115) can be used in ELISA,FCM,WB.
Pack Size | Price | Availability | Quantity |
---|---|---|---|
50 μL | $207 | 7-10 days | |
100 μL | $347 | 7-10 days |
Description | Anti-GOLM1 Antibody (1M115) is an antibody targeting GOLM1. Anti-GOLM1 Antibody (1M115) can be used in ELISA,FCM,WB. |
Ig Type | Rabbit IgG |
Clone | 1M115 |
Reactivity | Human |
Verified Activity | 1. Western Blot -Positive WB detected in: SH-SY5Y whole cell lysate, HepG2 whole cell lysate -All lanes: GOLM1 antibody at 1.3μg/ml -Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution -Predicted band size: 46, 45 KDa -Observed band size: 80 KDa 2. Overlay histogram showing Hela cells stained with TMAH-00499 (red line) at 1:50. The cells were fixed with 70% Ethylalcohol (18h) and then permeabilized with 0.3% Triton X-100 for 2 min. The cells were then incubated in 1x PBS /10% normal goat serum to block non-specific protein-protein interactions followed by primary antibody for 1 h at 4°C. The secondary antibody used was FITC goat anti-rabbit IgG (H+L) at 1/200 dilution for 1 h at 4°C. Control antibody (green line) was used under the same conditions. Acquisition of >10,000 events was performed. |
Application | |
Recommended Dose | WB:1:500-1:5000. |
Antibody Type | Monoclonal |
Subcellular Localization | Golgi apparatus, cis-Golgi network membrane; Single-pass type II membrane protein. Note=Early Golgi. Cycles via the cell surface and endosomes upon lumenal pH disruption. |
Tissue Specificity | Widely expressed. Highly expressed in colon, prostate, trachea and stomach. Expressed at lower level in testis, muscle, lymphoid tissues, white blood cells and spleen. Predominantly expressed by cells of the epithelial lineage. Expressed at low level in n |
Construction | Recombinant Antibody |
Purification | Affinity-chromatography |
Appearance | Liquid |
Formulation | Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
Research Background | Unknown. Cellular response protein to viral infection. |
Conjucates | Unconjugated |
Immunogen | A synthetic peptide: Human GOLM1 |
Antigen Species | Human |
Gene Name | GOLM1 |
Gene ID | |
Uniprot ID | |
Biology Area | Signal Transduction |
Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
Transport | Shipping with blue ice. |
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