Antibody Type: Recombinant Monoclonal

Application: ELISA, WB, FC

Reactivity: Human

Rabbit IgG

1M115

Human

1. Western Blot
-Positive WB detected in: SH-SY5Y whole cell lysate, HepG2 whole cell lysate
-All lanes: GOLM1 antibody at 1.3μg/ml
-Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution
-Predicted band size: 46, 45 KDa
-Observed band size: 80 KDa
2. Overlay histogram showing Hela cells stained with TMAH-00499 (red line) at 1:50. The cells were fixed with 70% Ethylalcohol (18h) and then permeabilized with 0.3% Triton X-100 for 2 min. The cells were then incubated in 1x PBS /10% normal goat serum to block non-specific protein-protein interactions followed by primary antibody for 1 h at 4°C. The secondary antibody used was FITC goat anti-rabbit IgG (H+L) at 1/200 dilution for 1 h at 4°C. Control antibody (green line) was used under the same conditions. Acquisition of >10,000 events was performed.

ELISA, WB, FC

WB:1:500-1:5000.

Monoclonal

Unconjugated

A synthetic peptide: Human GOLM1

Human

51280

Signal Transduction