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Anti-METAP2 Antibody (5B549)
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Catalog No. TMAH-00750
Alias p67eIF2, p67, MNPEP, methionyl aminopeptidase 2, MAP2
Anti-METAP2 Antibody (5B549) is an antibody targeting METAP2. Anti-METAP2 Antibody (5B549) can be used in ELISA, IHC.
Anti-METAP2 Antibody (5B549)
Anti-METAP2 Antibody (5B549)
😃Good
Catalog No. TMAH-00750Alias p67eIF2, p67, MNPEP, methionyl aminopeptidase 2, MAP2
Anti-METAP2 Antibody (5B549) is an antibody targeting METAP2. Anti-METAP2 Antibody (5B549) can be used in ELISA, IHC.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 50 μL | $208 | 7-10 days | |
| 100 μL | $347 | 7-10 days |
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Product Introduction
Bioactivity
Related Conjugates and Formulations
Antigen Details
Chemical Properties
| Description | Antibody Type: Recombinant Rabbit Monoclonal Application: ELISA, IHC Reactivity: Human |
| Alias | p67eIF2, p67, MNPEP, methionyl aminopeptidase 2, MAP2 |
| Ig Type | Rabbit IgG |
| Clone | 5B549 |
| Reactivity | Human |
| Verified Activity | IHC image of TMAH-00750 diluted at 1:100 and staining in paraffin-embedded human tonsil tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB. |
| Application | ELISA, IHC |
| Recommended Dose | IHC:1:50-1:200. |
| Antibody Type | Monoclonal |
| Subcellular Localization | Cytoplasm. Note=About 30% of expressed METAP2 associates with polysomes. |
| Construction | Recombinant Antibody |
| Purification | Affinity-chromatography |
| Appearance | Liquid |
| Formulation | Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| Research Background | Cotranslationally removes the N-terminal methionine from nascent proteins. The N-terminal methionine is often cleaved when the second residue in the primary sequence is small and uncharged (Met-Ala-, Cys, Gly, Pro, Ser, Thr, or Val). The catalytic activity of human METAP2 toward Met-Val peptides is consistently two orders of magnitude higher than that of METAP1, suggesting that it is responsible for processing proteins containing N-terminal Met-Val and Met-Thr sequences in vivo. Protects eukaryotic initiation factor EIF2S1 from translation-inhibiting phosphorylation by inhibitory kinases such as EIF2AK2/PKR and EIF2AK1/HCR. Plays a critical role in the regulation of protein synthesis. |
| Conjucates | Unconjugated |
| Immunogen | A synthetic peptide: Human Methionine Aminopeptidase 2 |
| Antigen Species | Human |
| Gene ID | 10988 |
| Uniprot ID | |
| Biology Area | Metabolism, Signal transduction |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
Sci Citations
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For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . 
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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