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CD3D & CD3E Heterodimer Protein, Human, Recombinant (Flag & His)

Catalog No. TMPY-03745
Synonyms: CD3-DELTA, CD3d molecule, delta (CD3-TCR complex), CD3d molecule, δ (CD3-TCR complex), CD3-δ, IMD19, T3D

CD3D & CD3E Heterodimer Protein, Human, Recombinant (Flag & His) is expressed in HEK293 with Flag and His tag. The predicted molecular weight is 31.05 kDa. Accession number: P04234-1&P07766-1

All products from TargetMol are for Research Use Only. Not for Human or Veterinary or Therapeutic Use.
CD3D & CD3E Heterodimer Protein, Human, Recombinant (Flag & His)
Pack Size Availability Price/USD Quantity
50 μg In stock $ 386.00
100 μg 5 days $ 682.00
200 μg 5 days $ 1,200.00
500 μg 5 days $ 2,540.00
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Biological Description
Technical Params
Product Properties
Biological Information Measured by its binding ability in a functional ELISA. Immobilized human CD3D & CD3E Heterodimer Protein at 2 μg/ml (100 μl/well) can bind OKT3-mIgG2a . The EC50 of OKT3-mIgG2a is 7-28 ng/ml.

Description CD3D & CD3E Heterodimer Protein, Human, Recombinant (Flag & His) is expressed in HEK293 with Flag and His tag. The predicted molecular weight is 31.05 kDa. Accession number: P04234-1&P07766-1
Species Human
Expression System HEK293
Tag Flag,His
Accession Number P04234-1&P07766-1
Synonyms CD3-DELTA, CD3d molecule, delta (CD3-TCR complex), CD3d molecule, δ (CD3-TCR complex), CD3-δ, IMD19, T3D
Construction A DNA sequence encoding the extracellular domain (Met1-Ala105) of human CD3D (P04234-1) was fused with a flag tag at the C-terminus, constructed the plasmid 1; A DNA sequence encoding the extracellular domain (Met1-Asp126) of human CD3E (P07766-1) was fused with a polyhistidine tag at the C-terminus, constructed the plasmid 2. The two plasmids were co-expressed and the human CD3D/CD3E heterodimer was purified.
Protein Purity ≥ 95 % as determined by SDS-PAGE. ≥ 95 % as determined by SEC-HPLC.

Molecular Weight Approxiamtely 31.05 kDa
Endotoxin < 1.0 EU per μg of the protein as determined by the LAL method.
Formulation Lyophilized from sterile PBS, pH 7.4. Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0. 01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
Reconstitution A hardcopy of datasheet with reconstitution instructions is sent along with the products. Please refer to it for detailed information.
Stability & Storage

Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

Shipping

In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.

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Keywords

CD3D & CD3E Heterodimer Protein, Human, Recombinant (Flag & His) CD3-DELTA CD3d molecule, delta (CD3-TCR complex) CD3d molecule, δ (CD3-TCR complex) CD3d molecule, delta CD3-δ IMD 19 CD3-TCR complex IMD19 T3D IMD-19 recombinant recombinant-proteins proteins protein

 

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