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CD3D & CD3E Heterodimer Protein, Human, Recombinant (Flag & His)

Catalog No. TMPY-03745
Synonyms: CD3-DELTA, CD3d molecule, delta (CD3-TCR complex), CD3d molecule, δ (CD3-TCR complex), CD3-δ, IMD19, T3D

CD3D & CD3E Heterodimer Protein, Human, Recombinant (Flag & His) is expressed in HEK293 mammalian cells with Flag and His tag. The predicted molecular weight is 31.05 kDa and the accession number is P04234-1&P07766-1.

All products from TargetMol are for Research Use Only. Not for Human or Veterinary or Therapeutic Use.
CD3D & CD3E Heterodimer Protein, Human, Recombinant (Flag & His)
Pack Size Availability Price/USD Quantity
50 μg In stock $ 386.00
100 μg 5 days $ 682.00
200 μg 5 days $ 1,200.00
500 μg 5 days $ 2,540.00
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Biological Description
Technical Params
Product Properties
Biological Information Measured by its binding ability in a functional ELISA. Immobilized human CD3D & CD3E Heterodimer Protein at 2 μg/ml (100 μl/well) can bind OKT3-mIgG2a . The EC50 of OKT3-mIgG2a is 7-28 ng/ml.

Description CD3D & CD3E Heterodimer Protein, Human, Recombinant (Flag & His) is expressed in HEK293 mammalian cells with Flag and His tag. The predicted molecular weight is 31.05 kDa and the accession number is P04234-1&P07766-1.
Species Human
Expression System HEK293 Cells
Tag C-Flag, C-His
Accession Number P04234-1&P07766-1
Synonyms CD3-DELTA, CD3d molecule, delta (CD3-TCR complex), CD3d molecule, δ (CD3-TCR complex), CD3-δ, IMD19, T3D
Construction The extracellular domain (Met1-Ala105) of Human CD3D (P04234-1) was fused with a flag tag at the C-terminus, constructed the plasmid 1; The extracellular domain (Met1-Asp126) of Human CD3E (P07766-1) was fused with a polyhistidine tag at the C-terminus, constructed the plasmid 2. The two plasmids were co-expressed and the Human CD3D/CD3E heterodimer was purified.
Protein Purity ≥ 95 % as determined by SDS-PAGE. ≥ 95 % as determined by SEC-HPLC.

Molecular Weight 31.05 kDa (predicted)
Endotoxin < 1.0 EU/μg of the protein as determined by the LAL method.
Formulation Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH 7.4. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
Reconstitution A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.
Stability & Storage

It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at-80℃. For reconstituted protein solutions, the solution can be stored at -20°c to -80'c for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.

Shipping

In general, Lyophilized powders are shipping with blue ice.

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Keywords

CD3D & CD3E Heterodimer Protein, Human, Recombinant (Flag & His) CD3-DELTA CD3d molecule, delta (CD3-TCR complex) CD3d molecule, δ (CD3-TCR complex) CD3d molecule, delta CD3-δ IMD 19 CD3-TCR complex IMD19 T3D IMD-19 recombinant recombinant-proteins proteins protein

 

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