CD3D & CD3E Heterodimer Protein, Human, Recombinant (Flag & His) is expressed in HEK293 with Flag and His tag. The predicted molecular weight is 31.05 kDa. Accession number: P04234-1&P07766-1
Pack Size | Availability | Price/USD | Quantity |
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50 μg | In stock | $ 386.00 | |
100 μg | 5 days | $ 682.00 | |
200 μg | 5 days | $ 1,200.00 | |
500 μg | 5 days | $ 2,540.00 |
Biological Information |
Measured by its binding ability in a functional ELISA. Immobilized human CD3D & CD3E Heterodimer Protein at 2 μg/ml (100 μl/well) can bind OKT3-mIgG2a . The EC50 of OKT3-mIgG2a is 7-28 ng/ml.
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Description | CD3D & CD3E Heterodimer Protein, Human, Recombinant (Flag & His) is expressed in HEK293 with Flag and His tag. The predicted molecular weight is 31.05 kDa. Accession number: P04234-1&P07766-1 |
Species | Human |
Expression System | HEK293 |
Tag | Flag,His |
Accession Number | P04234-1&P07766-1 |
Synonyms | CD3-DELTA, CD3d molecule, delta (CD3-TCR complex), CD3d molecule, δ (CD3-TCR complex), CD3-δ, IMD19, T3D |
Construction | A DNA sequence encoding the extracellular domain (Met1-Ala105) of human CD3D (P04234-1) was fused with a flag tag at the C-terminus, constructed the plasmid 1; A DNA sequence encoding the extracellular domain (Met1-Asp126) of human CD3E (P07766-1) was fused with a polyhistidine tag at the C-terminus, constructed the plasmid 2. The two plasmids were co-expressed and the human CD3D/CD3E heterodimer was purified. |
Protein Purity |
≥ 95 % as determined by SDS-PAGE. ≥ 95 % as determined by SEC-HPLC.
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Molecular Weight | Approxiamtely 31.05 kDa |
Endotoxin | < 1.0 EU per μg of the protein as determined by the LAL method. |
Formulation | Lyophilized from sterile PBS, pH 7.4. Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0. 01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA. |
Reconstitution | A hardcopy of datasheet with reconstitution instructions is sent along with the products. Please refer to it for detailed information. |
Stability & Storage |
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. |
Shipping |
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. |
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Please read the User Guide of Recombinant Proteins for more specific information.
CD3D & CD3E Heterodimer Protein, Human, Recombinant (Flag & His) CD3-DELTA CD3d molecule, delta (CD3-TCR complex) CD3d molecule, δ (CD3-TCR complex) CD3d molecule, delta CD3-δ IMD 19 CD3-TCR complex IMD19 T3D IMD-19 recombinant recombinant-proteins proteins protein