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CD3D & CD3E Heterodimer Protein, Human, Recombinant (Flag & His)
(Synonyms: T3D, IMD19, CD3-δ, CD3-DELTA, CD3d molecule, δ (CD3-TCR complex), CD3d molecule, delta (CD3-TCR complex)) Copy Product InfoSynonyms: T3D, IMD19, CD3-δ, CD3-DELTA, CD3d molecule, δ (CD3-TCR complex), CD3d molecule, delta (CD3-TCR complex)
Catalog No. TMPY-03745 Copy Product Info
CD3D & CD3E Heterodimer Protein, Human, Recombinant (Flag & His) is expressed in HEK293 mammalian cells with Flag and His tag. The predicted molecular weight is 31.05 kDa and the accession number is P04234-1&P07766-1.
| Pack Size | Price | USA Stock | Global Stock | Quantity |
|---|---|---|---|---|
| 5 µg | $75 | - | In Stock | |
| 10 µg | $118 | - | In Stock | |
| 20 µg | $196 | - | In Stock | |
| 50 µg | $386 | - | In Stock | |
| 100 µg | $682 | 7-10 days | 7-10 days | |
| 200 µg | $1,200 | 7-10 days | 7-10 days | |
| 500 µg | $2,530 | 7-10 days | 7-10 days |
For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)
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For research use only—not for human use. No sales to individuals. Use as intended only.
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Purity:SDS-PAGE: 98.1%; SEC-HPLC: 96.9%
Appearance:Lyophilized powder
Color:White
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Product Information
Bioactivity
Chemical Properties
Storage & Solubility Information
| Bioactivity | Measured by its binding ability in a functional ELISA. Immobilized human CD3D & CD3E Heterodimer Protein at 2 μg/ml (100 μl/well) can bind OKT3-mIgG2a . The EC50 of OKT3-mIgG2a is 7-28 ng/ml. |
| Description | CD3D & CD3E Heterodimer Protein, Human, Recombinant (Flag & His) is expressed in HEK293 mammalian cells with Flag and His tag. The predicted molecular weight is 31.05 kDa and the accession number is P04234-1&P07766-1. |
| Species | Human |
| Expression System | HEK293 Cells |
| Tag | C-Flag, C-His |
| Accession Number | P04234-1&P07766-1 |
| Construction | A DNA sequence encoding the extracellular domain (Met1-Ala105) of human CD3D (P04234-1) was fused with a flag tag at the C-terminus, constructed the plasmid 1; A DNA sequence encoding the extracellular domain (Met1-Asp126) of human CD3E (P07766-1) was fused with a polyhistidine tag at the C-terminus, constructed the plasmid 2. The two plasmids were co-expressed and the human CD3D/CD3E heterodimer was purified. Predicted N terminal: Phe 22 & Asp 23 |
| Protein Purity | ≥ 95 % as determined by SDS-PAGE. ≥ 95 % as determined by SEC-HPLC.  , |
| Endotoxin | < 1.0 EU/μg of the protein as determined by the LAL method. |
| Formulation | Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH 7.4. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization. |
| Reconstitution | Reconstituted with sterile deionized water to 0.25 mg/mL. Reconstitution conditions may vary depending on the lot. |
| Synonyms | T3D, IMD19, CD3-δ, CD3-DELTA, CD3d molecule, δ (CD3-TCR complex), CD3d molecule, delta (CD3-TCR complex) |
| Molecular Weight | 31.05 kDa (predicted); 20, 22 and 26 kDa (reducing condition, due to glycosylation) |
| Shipping | In general, lyophilized powders are shipped with blue ice, while solutions are shipped with dry ice. |
| Storage | It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots. |
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Related Tags: CD3D & CD3E Heterodimer Protein, Human, Recombinant (Flag & His) chemical structure | CD3D & CD3E Heterodimer Protein, Human, Recombinant (Flag & His) molecular weight
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