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Nectin-4 Protein, Human, Recombinant (His & Avi)

(Synonyms: PVRL4, PRR4, NECTIN4, LNIR, EDSS1) Copy Product Info

Synonyms: PVRL4, PRR4, NECTIN4, LNIR, EDSS1

Catalog No. TMPK-00308 Copy Product Info
Nectin-4 seems to be involved in cell adhesion through trans-homophilic and -heterophilic interactions, the latter including specifically interactions with NECTIN1. Does not act as receptor for alpha-herpesvirus entry into cells.(Microbial infection) Acts as a receptor for measles virus. Nectin-4 Protein, Human, Recombinant (His & Avi) is expressed in HEK293 mammalian cells with C-His-Avi tag. The predicted molecular weight is 37.2 kDa and the accession number is Q96NY8.

Batch Information

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Purity:> 95% as determined by Tris-Bis PAGE; > 95% as determined by SEC-HPLC
Appearance:Lyophilized powder
Color:White
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Product Introduction

Bioactivity
Bioactivity
Immobilized Human Nectin-4 at 0.5μg/ml (100μl/well) on the plate. Dose response curve for Anti-Nectin-4 Antibody, hFc Tag with the EC50 of 8.0ng/ml determined by ELISA.
Description
Nectin-4 seems to be involved in cell adhesion through trans-homophilic and -heterophilic interactions, the latter including specifically interactions with NECTIN1. Does not act as receptor for alpha-herpesvirus entry into cells.(Microbial infection) Acts as a receptor for measles virus. Nectin-4 Protein, Human, Recombinant (His & Avi) is expressed in HEK293 mammalian cells with C-His-Avi tag. The predicted molecular weight is 37.2 kDa and the accession number is Q96NY8.
Species
Human
Expression System
HEK293 Cells
TagC-His-Avi
Accession NumberQ96NY8
ConstructionGly32-Val351
Protein Purity
> 95% as determined by Tris-Bis PAGE; > 95% as determined by SEC-HPLC
Endotoxin< 1.0 EU/μg of the protein as determined by the LAL method.
FormulationLyophilized from a solution filtered through a 0.22 μm filter, containing PBS (pH 7.4). Typically, 8% trehalose is incorporated as a protective agent before lyophilization.
ReconstitutionReconstitute the lyophilized protein in distilled water. The product concentration should not be less than 100 μg/ml. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing.
SynonymsPVRL4, PRR4, NECTIN4, LNIR, EDSS1
Research Background
Nectin-4 seems to be involved in cell adhesion through trans-homophilic and -heterophilic interactions, the latter including specifically interactions with NECTIN1. Does not act as receptor for alpha-herpesvirus entry into cells.(Microbial infection) Acts as a receptor for measles virus.
Chemical Properties
Molecular Weight37.2 kDa (predicted). Due to glycosylation, the protein migrates to 46-48 kDa based on Tris-Bis PAGE result.
Storage & Solubility Information
ShippingIn general, lyophilized powders are shipped with blue ice, while solutions are shipped with dry ice.
StorageIt is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.

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Keywords

Related Tags: Nectin-4 Protein, Human, Recombinant (His & Avi) chemical structure | Nectin-4 Protein, Human, Recombinant (His & Avi) molecular weight