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Anti-Beta-galactosidase Antibody (5K148) (Synonyms: β-galactosidase Protein, β-galactosidase, β-gal, lacZ, L...)
Catalog No. TMAH-00680 Copy Product Info
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Anti-Beta-galactosidase Antibody (5K148) is a Mouse antibody targeting Beta-galactosidase. Anti-Beta-galactosidase Antibody (5K148) can be used in ELISA,FCM,IF,IP,WB.
Anti-Beta-galactosidase Antibody (5K148)
Copy Product Info🥰Excellent
Catalog No. TMAH-00680
Synonyms β-galactosidase Protein, β-galactosidase, β-gal, lacZ, Lactase, Beta-galactosidase, Beta-gal
Anti-Beta-galactosidase Antibody (5K148) is a Mouse antibody targeting Beta-galactosidase. Anti-Beta-galactosidase Antibody (5K148) can be used in ELISA,FCM,IF,IP,WB.
Anti-Beta-galactosidase Antibody
(5K148)
(5K148)
| Pack Size | Price | USA Stock | Global Stock | Quantity |
|---|---|---|---|---|
| 50 μL | $208 | 7-10 days | 7-10 days | |
| 100 μL | $349 | 7-10 days | 7-10 days |
For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)
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For research use only—not for human use. No sales to individuals. Use as intended only.
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Product Introduction
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| Description | Anti-Beta-galactosidase Antibody (5K148) is a Mouse antibody targeting Beta-galactosidase. Anti-Beta-galactosidase Antibody (5K148) can be used in ELISA,FCM,IF,IP,WB. |
| Synonyms | β-galactosidase Protein, β-galactosidase, β-gal, lacZ, Lactase, Beta-galactosidase, Beta-gal |
| Ig Type | IgG1 |
| Clone | 5K148 |
| Reactivity | Escherichia coli |
| Verified Activity | 1. Western Blot -Positive WB detected in Recombinant protein -All lanes: lacZ antibody at 1:2000, 1:4000, 1:8000, 1:16000, 1:32000, 1:64000 -Secondary: Goat polyclonal to Mouse IgG at 1/50000 dilution -Predicted band size: 117 kDa -Observed band size: 130 kDa 2. Western Blot -Positive WB detected in Recombinant protein (10ng, 5ng, 2.5ng, 1.25ng, 0.625ng) -All lanes: lacZ antibody at 1:2000 -Secondary: Goat polyclonal to Mouse IgG at 1/50000 dilution -Predicted band size: 117 kDa -Observed band size: 130 kDa 3. Immunofluorescence staining of 293T cells transfected with TMAH-00680 at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). The image on the right is the 293T cells transfected without lacZ antibody 4. Immunoprecipitating lacZ in 293T transfected whole cell lysate -Lane 1: Mouse control IgG instead of TMAH-00680 in 293T transfected whole cell lysate -Lane 2: TMAH-00680 (2µl) + 293T transfected whole cell lysate (500µg) -Lane 3: 293T transfected whole cell lysate (20µg) For western blotting, the blot was detected with Flag Tag Monoclonal Antibody at 1:2000, and a HRP-conjugated Protein G antibody was used as the secondary antibody at 1:2000 5. Overlay histogram showing 293T transfected cells (red line) or untransfected cells (green line) stained with TMAH-00680 at 1:200. The cells were incubated in 1x PBS /10% normal goat serum to block non-specific protein-protein interactions followed by primary antibody for 1 h at 4°C. The secondary antibody used was FITC goat anti-mouse IgG(H+L) at 1/200 dilution for 1 h at 4°C. |
| Application | |
| Antibody Type | Monoclonal |
| Host Species | Mouse |
| Construction | Hybridoma Monoclonal Antibody |
| Purification | Protein A purified |
| Appearance | Liquid |
| Formulation | Preservative: 0.03% Proclin 300. Constituents: 50% Glycerol, 0.01M PBS, PH 7.4. |
| Purity | >95% |
| Conjucates | Unconjugated |
| Immunogen | Recombinant Protein: Escherichia coli Beta-galactosidase Protein |
| Antigen Species | Escherichia coli |
| Gene Name | lacZ |
| Uniprot ID | |
| Biology Area | Tags & Cell Markers |
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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