Antibody Type: Mouse Monoclonal

Application: ELISA, WB, IF, IP, FCM

Reactivity: Escherichia coli

IgG1

5K148

Escherichia coli

1. Western Blot
-Positive WB detected in Recombinant protein
-All lanes: lacZ antibody at 1:2000, 1:4000, 1:8000, 1:16000, 1:32000, 1:64000
-Secondary: Goat polyclonal to Mouse IgG at 1/50000 dilution
-Predicted band size: 117 kDa
-Observed band size: 130 kDa
2. Western Blot
-Positive WB detected in Recombinant protein (10ng, 5ng, 2.5ng, 1.25ng, 0.625ng)
-All lanes: lacZ antibody at 1:2000
-Secondary: Goat polyclonal to Mouse IgG at 1/50000 dilution
-Predicted band size: 117 kDa
-Observed band size: 130 kDa
3. Immunofluorescence staining of 293T cells transfected with TMAH-00680 at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). The image on the right is the 293T cells transfected without lacZ antibody
4. Immunoprecipitating lacZ in 293T transfected whole cell lysate
-Lane 1: Mouse control IgG instead of TMAH-00680 in 293T transfected whole cell lysate
-Lane 2: TMAH-00680 (2µl) + 293T transfected whole cell lysate (500µg)
-Lane 3: 293T transfected whole cell lysate (20µg)
For western blotting, the blot was detected with Flag Tag Monoclonal Antibody at 1:2000, and a HRP-conjugated Protein G antibody was used as the secondary antibody at 1:2000
5. Overlay histogram showing 293T transfected cells (red line) or untransfected cells (green line) stained with TMAH-00680 at 1:200. The cells were incubated in 1x PBS /10% normal goat serum to block non-specific protein-protein interactions followed by primary antibody for 1 h at 4°C. The secondary antibody used was FITC goat anti-mouse IgG(H+L) at 1/200 dilution for 1 h at 4°C.

ELISA, WB, IF, IP, FCM

Monoclonal

Unconjugated

Recombinant Protein: Escherichia coli Beta-galactosidase Protein

Escherichia coli

Tags & Cell Markers