1.Immobilized SP34-derived anti-CD3E antibody, human IgG1, Human IgG1 at 2 μg/mL (100 μL/well) can bind Recombinant Rhesus CD3e/CD3 epsilon Protein (His Tag) (Cat#TMPY-02701), the EC50 is 1.0-3.0 ng/mL(QC tested).
2.anti-CD20/CD3 (Mosunetuzumab Biosimilar) captured on Protein A chip, can bind Recombinant Rhesus CD3e/CD3 epsilon Protein (His Tag) (Cat#TMPY-02701) with an affinity constant of 0.381 nM as determined in an SPR assay (Biacore T200) (Routinely tested).

CD3 epsilon/CD3e Protein, Rhesus, Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 12.3 kDa and the accession number is G7NCB9.

Rhesus

HEK293 Cells

CD3e molecule, ε (CD3-TCR complex),CD3e molecule, epsilon (CD3-TCR complex),CD3 ε/CD3e

A DNA sequence encoding the rhesus CD3E (XP_001097204.1) CD3E extracellular domain (Met 1-Asp 117) was fused with a polyhistidine tag at the C-terminus. Predicted N terminal: Gln 22

≥ 96 % as determined by SDS-PAGE. ≥ 90 % as determined by SEC-HPLC.

Reconstituted with sterile deionized water to 0.25 mg/mL. Reconstitution conditions may vary depending on the lot.

It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.

T-cell surface glycoprotein CD3 epsilon chain, also known as CD3E, is a single-pass type I membrane protein. CD3E contains 1 Ig-like (immunoglobulin-like) domain and 1 ITAM domain. CD3E, together with CD3-gamma, CD3-delta and CD3-zeta, and the T-cell receptor alpha/beta and gamma/delta heterodimers, forms the T cell receptor-CD3 complex. The CD3 epsilon subunit of the T cell receptor (TCR) complex contains two defined signaling domains, a proline-rich sequence and an immune tyrosine activation motifs (ITAMs), and this complex undergoes a conformational change upon ligand binding that is thought to be important for the activation of T cells. In the CD3 epsilon mutant mice, all stages of T cell development and activation that are TCR-dependent were impaired, but not eliminated, including activation of mature naïve T cells with the MHCII presented superantigen, staphylococcal enterotoxin B, or with a strong TCR cross-linking antibody specific for either TCR-Cbeta or CD3 epsilon. T cell receptor-CD3 complex plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways. This complex is critical for T-cell development and function, and represents one of the most complex transmembrane receptors. CD3E plays an essential role in T-cell development, and defects in CD3E gene cause severe immunodeficiency. Homozygous mutations in CD3D and CD3E genes lead to a complete block in T-cell development and thus to an early-onset severe combined immunodeficiency phenotype.Cancer ImmunotherapyImmune CheckpointImmunotherapyTargeted Therapy