Immobilized Human PD-1, mFc Tag at 1 μg/ml (100 μl/well) on the plate. Dose response curve for Human PD-L1, hFc Tag with the EC50 of 0.17 μg/ml determined by ELISA (QC Test).
Human PD-L1, hFc Tag captured on CM5 Chip via Protein A can bind Biotinylated Human PD-1, His-Avi Tag with an affinity constant of 2.356 μM as determined in SPR assay (Biacore T200).

PD-L1 Protein, Human, Recombinant (hFc) V2 is expressed in HEK293 Cells. The accession number is Q9NZQ7-1.

Human

HEK293 Cells

PD-L1,PDL1,PDCD1LG1,PDCD1L1,CD274 molecule,B7-H1,B7H1,B7-H

Phe19-Arg238

> 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC

Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.

B7-H1, also known as PD-L1 and CD274, is an approximately 65 kDa transmembrane glycoprotein in the B7 family of immune regulatory molecules. PD-L1 has been identified as the ligand for the immunoinhibitory receptor programmed death-1(PD1/PDCD1) and has been demonstrated to play a role in the regulation of immune responses and peripheral tolerance.