Immobilized Human PD-1, mFc Tag at 1 μg/ml (100 μl/well) on the plate. Dose response curve for Human PD-L1, hFc Tag with the EC50 of 0.17 μg/ml determined by ELISA (QC Test).
Human PD-L1, hFc Tag captured on CM5 Chip via Protein A can bind Biotinylated Human PD-1, His-Avi Tag with an affinity constant of 2.356 μM as determined in SPR assay (Biacore T200).

PD-L1 Protein, Human, Recombinant (hFc) V2 is expressed in HEK293 Cells. The accession number is Q9NZQ7-1.

Human

HEK293 Cells

PD-L1,PDL1,PDCD1LG1,PDCD1L1,CD274 molecule,B7-H1,B7H1,B7-H

Phe19-Arg238

> 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC

Reconstitute the lyophilized protein in sterile deionized water. The product concentration should not be less than 100 μg/mL. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing.

Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.

B7-H1, also known as PD-L1 and CD274, is an approximately 65 kDa transmembrane glycoprotein in the B7 family of immune regulatory molecules. PD-L1 has been identified as the ligand for the immunoinhibitory receptor programmed death-1(PD1/PDCD1) and has been demonstrated to play a role in the regulation of immune responses and peripheral tolerance.