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Neuropilin-1 Protein, Human, Recombinant (isoform b, His)

Catalog No. TMPY-02367
Synonyms: neuropilin 1, NRP, VEGF165R, BDCA4, Neuropilin-1, NP1, CD304

Neuropilin-1 Protein, Human, Recombinant (isoform b, His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 71.3 kDa and the accession number is O14786-2.

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Neuropilin-1 Protein, Human, Recombinant (isoform b, His)
Pack Size Availability Price/USD Quantity
50 μg In stock $ 263.00
100 μg 5 days $ 423.00
200 μg 5 days $ 680.00
500 μg 5 days $ 1,270.00
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Biological Description
Technical Params
Product Properties
References and Literature
Biological Information 1. Using the Octet RED System, the affinity constant (Kd) of NRP1-his bound to biotinylated human VEGF165 was 25nM. 2. Immobilized Recombinant Human / Cynomolgus VEGF / VEGFA / VEGF165 Protein at 2 μg/mL (100 μL/well) can bind Recombinant Human Neuropilin-1 / NRP1 / CD304 Protein (His Tag) with a linear range of 32-160 μg/mL.

Description Neuropilin-1 Protein, Human, Recombinant (isoform b, His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 71.3 kDa and the accession number is O14786-2.
Species Human
Expression System HEK293 Cells
Tag C-His
Accession Number O14786-2
Synonyms neuropilin 1, NRP, VEGF165R, BDCA4, Neuropilin-1, NP1, CD304
Construction The Human NRP1 isoform b (NP_001019799.2, with natural variants V179A) (Met 1-Lys 644) was fused with a polyhistidine tag at the C-terminus.
Protein Purity ≥ 95 % as determined by SDS-PAGE. ≥ 95 % as determined by SEC-HPLC.

Molecular Weight 71.3 kDa (predicted)
Endotoxin < 1.0 EU/μg of the protein as determined by the LAL method.
Formulation Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH 7.4. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
Reconstitution A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.
Stability & Storage

It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at-80℃. For reconstituted protein solutions, the solution can be stored at -20°c to -80'c for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.

Shipping

In general, Lyophilized powders are shipping with blue ice.

Research Background Neuropilin is a type I transmembrane protein and the molecular mass is 120 kDa. Two homologs, Neuropilin-1 and Neuropilin-2, are identified. The primary structure of Neuropilin-1 and Neuropilin-2 is well conserved and is divided into four domains, CUB (a1/a2) domain, FV/FVIII (b1/b2) domain, MAM (c) domain, and (d) domain that contains a transmembrane and a short cytoplasmic region. Neuropilin-1 (NRP1) acts as a receptor for two different extracellular ligands, class 3 semaphorins, and specific isoforms of vascular endothelial growth factor. The functions of NRP1 and NRP2 have been extensively studied in neurons where they act in axon guidance and in endothelial cells where they promote angiogenesis and cell migration. Neuropilin-1 is likely to mediate contacts between the dendritic cells and the T lymphocytes via homotypic interactions and is essential for the initiation of the primary immune response. NRP1 is a co-receptor for VEGF receptor-2 (VEGFR2) that enhances the binding of VEGF165 to VEGFR2 and VEGF165-mediated chemotaxis. NRP1 expression is regulated in EC by tumor necrosis factor-alpha, the transcription factors dHAND and Ets-1, and vascular injury. NRP1 upregulation is positively correlated with the progression of various tumors. Overexpression of NRPI in rat tumor cells results in enlarged tumors and substantially enhanced tumor angiogenesis. On the other hand, soluble NRP1 (sNRP1) is an antagonist of tumor angiogenesis.

References and Literature

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Keywords

Neuropilin-1 Protein, Human, Recombinant (isoform b, His) CD-304 NP-1 neuropilin 1 NP 1 NRP CD 304 VEGF165R BDCA4 Neuropilin-1 BDCA-4 NP1 BDCA 4 CD304 recombinant recombinant-proteins proteins protein

 

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