BAFF/TNFSF13B Protein, Human, Recombinant (His)

Name: BAFF/TNFSF13B Protein, Human, Recombinant (His)
Brand: TargetMol
SKU: TMPJ-00178
Rating: 4.5 (1 reviews)

(Synonyms: Tumor necrosis factor ligand superfamily member 13B, TNF- and APOL-related leukocyte expressed ligand 1, TALL-1, Dendritic cell-derived TNF-like molecule, BLyS, B-cell-activating factor, BAFF, B lymphocyte stimulator) Copy Product Info

Synonyms: Tumor necrosis factor ligand superfamily member 13B, TNF- and APOL-related leukocyte expressed ligand 1, TALL-1, Dendritic cell-derived TNF-like molecule, BLyS, B-cell-activating factor, BAFF, B lymphocyte stimulator

Catalog No. TMPJ-00178 Copy Product Info

BAFF/TNFSF13B Protein, Human, Recombinant (His) is expressed in HEK293 mammalian cells with N-6xHis tag. The predicted molecular weight is 22 KDa and the accession number is Q9Y275.

Pack Size	Price	USA Stock	Global Stock
5 µg	$83	7-10 days	7-10 days
10 µg	$133	7-10 days	7-10 days
20 µg	$213	7-10 days	7-10 days
50 µg	$403	7-10 days	7-10 days
100 µg	$673	7-10 days	7-10 days
200 µg	$1,120	7-10 days	7-10 days
500 µg	$2,230	7-10 days	7-10 days
1 mg	$3,350	7-10 days	7-10 days

For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)

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For research use only—not for human use. No sales to individuals. Use as intended only.

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Purity:Greater than 95% as determined by reducing SDS-PAGE.

Appearance:Lyophilized powder

Color:White

Resource Download

COA User Guide of Recombinant Proteins

Product Introduction

Bioactivity

Bioactivity	1. Loaded Human BCMA-Fc on Protein A Biosensor, can bind Human BAFF-His with an affinity constant of 0.02 nM as determined in BLI assay. (Regularly tested)2 . Immobilized Human BAFF-His at 10μg/ml (100 μl/well) can bind Mouse BCMA-Fc . The ED50 of Recombinant Mouse BCMA-Fc is 0.016 ug/ml. (Regularly tested)
Description	BAFF/TNFSF13B Protein, Human, Recombinant (His) is expressed in HEK293 mammalian cells with N-6xHis tag. The predicted molecular weight is 22 KDa and the accession number is Q9Y275.
Species	Human
Expression System	HEK293 Cells
Tag	N-6xHis
Accession Number	Q9Y275
Amino Acid	Ala134-Leu285
Construction	Ala134-Leu285
Protein Purity	Greater than 95% as determined by reducing SDS-PAGE. (QC verified)
Endotoxin	< 0.1 ng/µg (1 EU/µg) as determined by LAL test.
Formulation	Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH 7.4.
Reconstitution	Reconstitute the lyophilized protein in distilled water. The product concentration should not be less than 100 μg/ml. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing.
Synonyms	Tumor necrosis factor ligand superfamily member 13B, TNF- and APOL-related leukocyte expressed ligand 1, TALL-1, Dendritic cell-derived TNF-like molecule, BLyS, B-cell-activating factor, BAFF, B lymphocyte stimulator
Research Background	TNFSF13B/TNFSF20 belongs to the tumor necrosis factor family. It abundantly is expressed in peripheral blood Leukocytes and is specifically expressed in monocytes and macrophages. Also found in the spleen, lymph node, bone marrow, T-cells and dendritic cells. A lower expression seen in placenta, heart, lung, fetal liver, thymus, and pancreas. Isoform 2 is expressed in many myeloid cell lines. Cytokine that binds to TNFRSF13B/TACI and TNFRSF17/BCMA. TNFSF13/APRIL binds to the same 2 receptors. Together, they form a 2 ligands -2 receptors pathway involved in the stimulation of B- and T-cell function and the regulation of humoral immunity. A third B-cell specific BAFF-receptor (BAFFR/BR3) promotes the survival of mature B-cells and the B-cell response. Isoform 2 seems to inhibit isoform 1 secretion and bioactivity. Isoform 3 acts as a transcription factor for its own parent gene, in association with NF-kappa-B p50 subunit, at least in autoimmune and proliferative B-cell diseases. The presence of Delta4BAFF is essential for soluble BAFF release by IFNG/IFN-gamma-stimulated monocytes and for B-cell survival. It can directly or indirectly regulate the differential expression of a large number of genes involved in the innate immune response and the regulation of apoptosis. Isoform 2 heteromultimerizes with isoform 1, probably limiting the amount of functional isoform 1 on the cell surface. Isoform 3 is unlikely form trimers or bind to BAFF receptors. Mature human BAFF consists of a 46 amino acid (aa) cytoplasmic domain, a 21 aa transmembrane segment, and a 218 aa extracellular domain (ECD) with a stalk region and one TNF-like domain. Within aa 134-285 of the ECD, human BAFF shares 72% aa sequence identity with mouse BAFF.

Chemical Properties

Molecular Weight

22 KDa (reducing condition)

Storage & Solubility Information

Shipping	In general, lyophilized powders are shipped with blue ice, while solutions are shipped with dry ice.
Storage	Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.