1. Measured in a cell proliferation assay using HUVEC human umbilical vein endothelial cells. The ED50 for this effect is typically 2-8 ng/mL. 2. Immobilized Cynomolgus mFc-TNFSF12 at 10 μg/ml (100 μl/well) can bind human Fc-TNFRSF12A , The EC50 of human Fc-TNFRSF12A is 0.07-0.15 μg/ml.

TWEAK/TNFSF12 Protein, Human, Cynomolgus, Recombinant (mFc) is expressed in HEK293 mammalian cells with mFc tag. The predicted molecular weight is 43.8 kDa and the accession number is F7HGN4.

Human,Cynomolgus

HEK293 Cells

tumor necrosis factor (ligand) superfamily, member 12

A DNA sequence encoding the human / cynomolgus TNFSF12 (F7HGN4) (Ser94-His249) was expressed with the Fc region of mouse IgG1 at the N-terminus. Human and Cynomolgus TNFSF12 sequences are identical. Predicted N terminal: Asp

> 95 % as determined by SDS-PAGE

A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.

It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.

TNFSF12 is a cytokine that belongs to the tumor necrosis factor (TNF) ligand family. It is a ligand for the FN14/TWEAKR receptor. TNFSF12 has overlapping signaling functions with TNF, but displays a much wider tissue distribution. It can induce apoptosis via multiple pathways of cell death in a cell type-specific manner. It is also found that TNFSF12 promotes proliferation and migration of endothelial cells, and thus acts as a regulator of angiogenesis. TNFSF12 also is a weak inducer of apoptosis in some cell types and mediates NF-kappa-B activation.Cancer ImmunotherapyImmune CheckpointImmunotherapyTargeted Therapy